State Key Laboratory of Veterinary Biotechnology, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences, 678 Haping Road, Harbin, 150069, People's Republic of China.
College of Life Science and Technology, Mudanjiang Normal University, 191 Wenhua Street, Mudanjiang, 157011, People's Republic of China.
Virol J. 2018 Feb 1;15(1):27. doi: 10.1186/s12985-018-0935-5.
Goose parvovirus (GPV) causes acute enteritis, hepatitis, myocarditis and high morbidity and mortality in geese and ducks. GPV H strain was isolated from a Heilongjiang goose farm where the geese were showing signs of hemorrhage in the brain, liver, and intestinal tract. In this study, we explored the genetic diversity among waterfowl parvovirus isolates and the pathological characteristics of GPV H in Shaoxing ducklings.
The complete capsid protein (VP) and non-structural (NS) sequences of the isolated H strain were sequenced, and phylogenetic trees of VP and NS were constructed in MEGA version 5.05 using the neighbor-joining method. Three-day-old Shaoxing ducklings were inoculated with GPV and were euthanized at 1, 2, 4, 6, and 8 days post-inoculation (PI), and their organs were removed and collected. The organs of 6-day PI ducklings were fixed in formalin, embedded in paraffin, sectioned for histology, stained with HE and analyzed for pathological lesions. The distribution of the GPV H strain in the tissues of the inoculated ducklings was detected using the polymerase chain reaction (PCR) method.
Genetic analysis of the NS and VP genes indicated that the H strain was closely related to strains circulating in China during 1999-2014, and the nucleic acid identity of those strains was 98%-99%. Classical symptoms were observed in the inoculated ducklings. GPV remained in many tissues and replicated in a majority of the tissues, leading to histopathological lesions in four tissues.
We first reported the distribution and histopathological lesions of a Chinese strain of GPV in infected shaoxing ducklings. This H strain was moderate pathogenic for Shaoxing ducklings.
鹅细小病毒（GPV）可引起鹅和鸭的急性肠炎、肝炎、心肌炎，发病率和死亡率较高。从黑龙江某鹅场出现脑、肝和肠道出血症状的鹅群中分离到了 GPV H 株。本研究旨在探讨水禽细小病毒分离株的遗传多样性及 GPV H 株在绍兴鸭中的致病性。
对分离的 H 株进行完整衣壳蛋白（VP）和非结构（NS）序列测序，采用 MEGA 5.05 软件中的邻接法构建 VP 和 NS 基因的系统进化树。3 日龄绍兴鸭接种 GPV 后，于接种后 1、2、4、6 和 8 天安乐处死，采集各组织器官。6 日龄 PI 鸭的组织器官经福尔马林固定、石蜡包埋、切片、HE 染色，观察病理变化。采用聚合酶链反应（PCR）法检测接种鸭各组织中 GPV H 株的分布。
NS 和 VP 基因遗传分析表明，H 株与 1999-2014 年中国流行株亲缘关系密切，核酸同源性为 98%-99%。接种鸭出现典型临床症状。GPV 广泛分布于各组织中，并在多数组织中复制，导致 4 种组织出现组织病理学损伤。
本研究首次报道了中国 GPV 株在感染绍兴鸭中的分布和组织病理学损伤。该 H 株对绍兴鸭呈中等致病性。
