Chen Shilong, Wang Shao, Cheng Xiaoxia, Xiao Shifeng, Zhu Xiaoli, Lin Fengqiang, Wu Nanyang, Wang Jinxiang, Huang Meiqing, Zheng Min, Chen Shaoying, Yu Fusong
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Arch Virol. 2016 Sep;161(9):2407-16. doi: 10.1007/s00705-016-2926-4. Epub 2016 Jun 17.
Many mule duck and Cherry Valley duck flocks in different duck-producing regions of China have shown signs of an apparently new disease designated "short beak and dwarfism syndrome" (SBDS) since 2015. The disease is characterized by dyspraxia, weight loss, a protruding tongue, and high morbidity and low mortality rates. In order to characterize the etiological agent, a virus designated SBDSV M15 was isolated from allantoic fluid of dead embryos following serial passage in duck embryos. This virus causes a cytopathic effect in duck embryo fibroblast (DEF) cells. Using monoclonal antibody diagnostic assays, the SBDSV M15 isolate was positive for the antigen of goose parvovirus but not Muscovy duck parvovirus. A 348-bp (2604-2951) VP1gene fragment was amplified, and its sequence indicated that the virus was most closely related to a Hungarian GPV strain that was also isolated from mule ducks with SBDS disease. A similar disease was reproduced by inoculating birds with SBDSV M15. Together, these data indicate that SBDSV M15 is a GPV-related parvovirus causing SBDS disease and that it is divergent from classical GPV isolates.
自2015年以来，中国不同鸭养殖地区的许多骡鸭和樱桃谷鸭群出现了一种明显的新疾病迹象，被称为“短喙和侏儒综合征”（SBDS）。该疾病的特征是运动障碍、体重减轻、舌头突出，发病率高且死亡率低。为了鉴定病原体，从鸭胚连续传代后的死胚尿囊液中分离出一种名为SBDSV M15的病毒。这种病毒在鸭胚成纤维细胞（DEF）中引起细胞病变效应。使用单克隆抗体诊断试验，SBDSV M15分离株对鹅细小病毒抗原呈阳性，但对番鸭细小病毒抗原呈阴性。扩增出一个348bp（2604-2951）的VP1基因片段，其序列表明该病毒与一株同样从患有SBDS疾病的骡鸭中分离出的匈牙利GPV毒株关系最为密切。用SBDSV M15接种禽类可再现类似疾病。这些数据共同表明，SBDSV M15是一种与GPV相关的细小病毒，可导致SBDS疾病，且与经典GPV分离株不同。
