ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA; Department of Pathology, University of Utah, 500 Chipeta Way, Salt Lake City, UT, 84108, USA.
Clin Chim Acta. 2018 May;480:71-78. doi: 10.1016/j.cca.2018.01.037. Epub 2018 Jan 31.
The present studies were conducted to characterize lipemic interference across three FDA-cleared ceruloplasmin (CERU) assays and to evaluate procedures designed to remove lipemic interference.
CERU assays on the Abbott ARCHITECT ci8200, Beckman AU5800, and Roche cobas Integra 400 Plus were evaluated. Precision, linearity with dilution, lipemic interference, and three methods for removing lipemia were assessed on each platform: ultracentrifugation (UC), lipemia-clearing reagent LipoClear (LC), and 1:5 dilution (DIL). Lipemia-index (L-index) thresholds were established using endogenously lipemic specimens and sera spiked with human-derived triglyceride-rich lipoproteins.
The ci8200 showed greater susceptibility to endogenous lipemic interference than would be expected based on vendor-derived limits established with Intralipid. Endogenous lipemia causes a negative interference on the ci8200 and a positive interference on the Integra. UC was generally the most reliable method of removing lipemic interference without impacting baseline CERU results.
CERU assays on different platforms have varying susceptibility to lipemic interference. L-index thresholds derived using Intralipid may not accurately represent interference caused by endogenous lipemia.
本研究旨在描述三种经 FDA 批准的铜蓝蛋白（CERU）检测试剂盒的脂血干扰特征，并评估用于去除脂血干扰的方法。
对 Abbott ARCHITECT ci8200、Beckman AU5800 和 Roche cobas Integra 400 Plus 上的 CERU 检测进行评估。在每个平台上评估精密度、稀释线性、脂血干扰以及三种去除脂血的方法：超速离心（UC）、脂血清除试剂 LipoClear（LC）和 1:5 稀释（DIL）。使用内源性脂血标本和用人源性甘油三酯丰富的脂蛋白加标血清建立脂血指数（L-index）阈值。
ci8200 对内源性脂血干扰的敏感性比根据 Intralipid 确定的供应商衍生限制所预期的要高。内源性脂血对 ci8200 产生负干扰，对 Integra 产生正干扰。UC 通常是去除脂血干扰而不影响基线 CERU 结果的最可靠方法。
不同平台上的 CERU 检测对脂血干扰的敏感性不同。使用 Intralipid 得出的 L-index 阈值可能无法准确表示内源性脂血引起的干扰。
Zotero 插件