Abaker Ismail A, Salih Diaeldin A, Haj Lima M El, Ahmed Rawia E, Osman Manal M, Ali Awadia M
Department of Parasitology, Faculty of Veterinary Science, University of Nyala, Nyala, Sudan.
Central Veterinary Research Laboratory, P.O. Box 8067, Al Amarat, Khartoum, Sudan.
Vet World. 2017 Dec;10(12):1475-1480. doi: 10.14202/vetworld.2017.1475-1480. Epub 2017 Dec 15.
This study was conducted in dairy cattle in Nyala, South Darfur State, during the period from June to September 2015, to study the prevalence of bovine tropical theileriosis.
Apparently, healthy cattle of different age groups, different breeds, and from both sexes were randomly selected from seven locations. Three age groups of cattle were selected, group one <1 year old, group two 1-3 years old, and group three older than 3 years. These cattle were indigenous and cross (Friesian X zebu). A total of 150 blood samples were collected for blood smears, blood in EDTA tubes, and serum samples as well as ticks infesting cattle. Three diagnostic techniques were used such as blood smear, indirect fluorescent antibody test (IFAT), and polymerase chain reaction (PCR).
Of 150 samples, 11 (7.3%, 95% confidence interval [CI]: 9.1-5.5) were positive for spp. piroplasms in the blood smears, 70 (46.7%, 95% CI: 35.7-57.7) were positive for antibodies in the IFAT, and of 100 samples, 39 (39%, 95% CI: 46.6-31.4) were positive for using PCR. The prevalence of was higher in indigenous breed than cross cattle by the three diagnostic techniques. The highest prevalence of was recorded among cattle older than 3 years old. There were three genera and ten species of ticks found feeding on cattle. These were , and .
The study concluded that tropical theileriosis is prevalent among dairy cattle in Nyala. was found in very low numbers, suggesting other ticks may play a role in the transmission of the disease. Molecular characterization of is recommended for accurate mapping of the disease and evaluates the magnitude problem of tropical theileriosis in South Darfur region.
本研究于2015年6月至9月期间在南达尔富尔州尼亚拉的奶牛中开展，以研究牛热带泰勒虫病的流行情况。
从七个地点随机选取不同年龄组、不同品种及不同性别的健康奶牛。选取了三个年龄组的奶牛，第一组小于1岁，第二组1 - 3岁，第三组大于3岁。这些奶牛包括本地品种以及杂交品种（弗里斯兰牛×瘤牛）。共采集了150份血样用于制作血涂片、采集EDTA抗凝管中的血液和血清样本，以及采集寄生在奶牛身上的蜱虫。使用了三种诊断技术，即血涂片检查、间接荧光抗体试验（IFAT）和聚合酶链反应（PCR）。
在150份样本中，血涂片检查发现11份（7.3%，95%置信区间[CI]：9.1 - 5.5）存在梨形虫属原虫阳性；IFAT检测发现70份（46.7%，95% CI：35.7 - 57.7）抗体阳性；在100份样本中，PCR检测发现39份（39%，95% CI：46.6 - 31.4）阳性。通过三种诊断技术，本地品种奶牛中梨形虫属原虫的流行率高于杂交奶牛。在大于3岁的奶牛中记录到最高的梨形虫属原虫流行率。发现有三个属和十个种的蜱虫寄生在奶牛身上。它们分别是 、 和 。
该研究得出结论，尼亚拉奶牛中热带泰勒虫病流行。发现梨形虫属原虫的数量非常少,表明其他蜱虫可能在该病传播中起作用。建议对梨形虫属原虫进行分子特征分析，以便准确绘制该病分布图并评估南达尔富尔地区热带泰勒虫病的严重程度问题。
