Ren Yujun, Miao Ying
Center for Molecular Cell and Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Methods Mol Biol. 2018;1744:247-265. doi: 10.1007/978-1-4939-7672-0_20.
Micro RNAs (miRNAs) are small noncoding RNA molecules that function in transcriptional level to regulate gene expression both in plants and animals. Increasing researches have shown that miRNAs are key regulators in plant development and stress responses, and emerging evidence indicates the potential role of miRNAs on plant senescence. In this chapter we summarize the daily methods used for identification and study of miRNAs in plants, including the isolation of total RNA, the purification of miRNAs, and the methods used to detect miRNAs in plants. The committed steps or modifications of these methods used in plant senescence research are noted.
微小RNA（miRNA）是一类小的非编码RNA分子，在植物和动物中发挥转录水平的基因表达调控作用。越来越多的研究表明，miRNA是植物发育和应激反应的关键调节因子，并且新出现的证据表明miRNA在植物衰老过程中具有潜在作用。在本章中，我们总结了用于鉴定和研究植物miRNA的日常方法，包括总RNA的分离、miRNA的纯化以及检测植物中miRNA的方法。文中还指出了植物衰老研究中这些方法的关键步骤或改进。
