Laboratório de Fisiopatologia, Instituto Butantan, São Paulo, Brazil; Programa de Pós-Graduação em Patologia Ambiental e Experimental, Universidade Paulista, São Paulo, Brazil.
Laboratório de Fisiopatologia, Instituto Butantan, São Paulo, Brazil.
Ticks Tick Borne Dis. 2018 Mar;9(3):519-525. doi: 10.1016/j.ttbdis.2018.01.004. Epub 2018 Jan 31.
The Gené's organ (GO) secretes a waxy substance on eggs that reduces water loss and has antimicrobial properties. The current study evaluated morphological and histochemical aspects of GO in Amblyomma sculptum from the period of post-feeding - when ticks detach from the host - to the stage just before oviposition. In this species, GO is composed of a corpus and two pairs of glands, namely, cranial and caudal. Glandular cells are joined laterally by a system of interdigitating membranes with junctional complexes. Histochemistry showed that lipid droplets became more evident as GO developed, while glycogen gradually disappeared, and proteins were detected only near the onset of oviposition. The ultrastructural results revealed a marked distension of the cuticle filled with an amorphous material. Glandular cells showed poor endoplasmatic reticulum, many mitochondria mainly in the basal cell poles and a very developed basal labyrinth. We concluded that the development of GO in A. sculptum ticks was continuous and progressive, and it started after detachment from the host. Additionally, the ultrastructure study suggests that gland cells have an important absorption ability and a low synthetic activity, which indicates that the majority of wax precursors are derived from haemolymph.
根纳氏器（GO）在卵上分泌一种蜡质物质，可减少水分流失并具有抗菌特性。本研究评估了 Amblyomma sculptum 在喂养后-当蜱从宿主上脱落-到产卵前阶段的 GO 的形态和组织化学方面。在该物种中，GO 由一个主体和两对腺体组成，即颅侧和尾侧。腺体细胞通过具有连接复合体的交错膜系统侧向连接。组织化学显示，随着 GO 的发育，脂滴变得更加明显，而糖原逐渐消失，并且仅在产卵开始时才检测到蛋白质。超微结构结果显示角质层明显膨胀，充满无定形物质。腺体细胞显示内质网不良，许多线粒体主要位于基底细胞极和非常发达的基底迷路。我们得出结论，A. sculptum 蜱的 GO 发育是连续和渐进的，并且在从宿主上脱落之后开始。此外，超微结构研究表明，腺细胞具有重要的吸收能力和较低的合成活性，这表明大多数蜡前体来自血淋巴。
