成纤维细胞中脆性位点的诱导
Induction of fragile sites in fibroblasts.
作者信息
Sutherland G R, Baker E
出版信息
Am J Hum Genet. 1986 Apr;38(4):573-5.
Abstract
A simple, reliable method for the induction of folate-sensitive fragile sites, including the fragile X, in fibroblasts is described. The method involves only the addition of 600 mg/l thymidine to cultures 24 hrs before they are harvested.
摘要
本文描述了一种在成纤维细胞中诱导包括脆性X在内的叶酸敏感性脆性位点的简单可靠方法。该方法仅需在收获培养物前24小时向培养物中添加600mg/l的胸腺嘧啶核苷。
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Induction of fragile sites in fibroblasts.成纤维细胞中脆性位点的诱导
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引用本文的文献
1
Fibroblast-specific common fragile sites induced by aphidicolin.
Hum Genet. 1989 Aug;83(1):45-8. doi: 10.1007/BF00274145.
2
Cell type-dependent difference in the distribution and frequency of excess thymidine-induced common fragile sites: T lymphocytes and skin fibroblasts.
Hum Genet. 1990 May;84(6):527-31. doi: 10.1007/BF00210803.
本文引用的文献
1
Expression in lymphocyte and fibroblast culture of the fragile X chromosome: a new technical approach.脆性X染色体在淋巴细胞和成纤维细胞培养中的表达：一种新的技术方法。
Hum Genet. 1981;59(2):166-9. doi: 10.1007/BF00293069.
2
A simple method to demonstrate the fragile X chromosome in fibroblasts.一种在成纤维细胞中显示脆性X染色体的简单方法。
Hum Genet. 1981;59(2):186. doi: 10.1007/BF00293076.
3
Marker X chromosome induction in fibroblasts by FUdR.氟尿苷（FUdR）诱导成纤维细胞中X染色体标记
Am J Med Genet. 1981;9(3):263-4. doi: 10.1002/ajmg.1320090313.
4
FUdR induction of the X chromosome fragile site: evidence for the mechanism of folic acid and thymidine inhibition.氟脱氧尿苷对X染色体脆性位点的诱导作用：叶酸和胸苷抑制机制的证据
Am J Hum Genet. 1981 Mar;33(2):234-42.
5
DNA polymerase alpha inhibition by aphidicolin induces gaps and breaks at common fragile sites in human chromosomes.阿非科林对DNA聚合酶α的抑制作用会在人类染色体的常见脆性位点诱导缺口和断裂。
Hum Genet. 1984;67(2):136-42. doi: 10.1007/BF00272988.
6
Excess thymidine induces folate sensitive fragile sites.
Am J Med Genet. 1985 Oct;22(2):433-43. doi: 10.1002/ajmg.1320220234.
7
First trimester prenatal diagnosis of a male fetus with fragile X.孕早期对患有脆性X综合征的男性胎儿进行产前诊断。
Lancet. 1985 Apr 13;1(8433):870. doi: 10.1016/s0140-6736(85)92227-5.
8
Heritable fragile sites on human chromosomes I. Factors affecting expression in lymphocyte culture.人类染色体上的遗传性脆性位点。I. 影响淋巴细胞培养中表达的因素。
Am J Hum Genet. 1979 Mar;31(2):125-35.
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