Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 08826, Republic of Korea.
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
Bioresour Technol. 2018 Apr;254:340-346. doi: 10.1016/j.biortech.2018.01.108. Epub 2018 Feb 4.
Vibrational wave treatment has been used to increase proliferation of microalgae. When directly applied at large scale, however, turbulence can offset positive effects of vibration on microalgae proliferation. Moreover, severe hydrodynamic shear fields in the bioreactor decrease cell viability that detrimentally influence maximum yieldable biomass. In this study, vibration pretreatment (between 10-30 Hz and 0.15-0.45 G) was used to prime the cells for enhanced biomass. When exposed to 10 Hz at 0.15 G for 72 h and inoculated in baffled flasks of large shear fields (0.292 Pa for the average wall shear force (aveWSF) and 184 s for the average shear strain rate (aveSSR)), microalgae showed 27% increase in biomass as well as 39% increase in corresponding amount of heterologous protein (i.e. GFP-3HA). Our results show that stress primed microalgae with vibrations can lead to improved proliferation that results in increased biomass production at industrial scale bioprocesses.
振动波处理已被用于增加微藻的增殖。然而，当直接大规模应用时，湍流会抵消振动对微藻增殖的积极影响。此外，生物反应器中剧烈的水动力剪切场会降低细胞活力，从而对最大可产生物质产生不利影响。在这项研究中，振动预处理（10-30 Hz 和 0.15-0.45 G）用于为增强生物质进行细胞预刺激。当在 10 Hz 和 0.15 G 下暴露 72 h 并接种到具有大剪切场的挡板瓶中（平均壁面剪切力 (aveWSF) 为 0.292 Pa，平均剪切应变速率 (aveSSR) 为 184 s）时，微藻的生物量增加了 27%，异源蛋白（即 GFP-3HA）的相应含量也增加了 39%。我们的结果表明，振动预刺激的应激微藻可以促进增殖，从而在工业规模的生物工艺中增加生物量的生产。
