Centre for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200135, China; Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200135, China.
Centre for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University; National Research Centre for Assisted Reproductive Technology and Reproductive Genetics; The Key laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education Shandong Provincial Key Laboratory of Reproductive Medicine, China.
Reprod Biomed Online. 2018 Apr;36(4):483-489. doi: 10.1016/j.rbmo.2017.12.020. Epub 2018 Jan 20.
Polycystic ovary syndrome (PCOS) is characterized by hyperandrogenism, ovarian dysfunction and polycystic ovarian morphology. Leukaemia inhibitory factor (LIF) affects many reproductive activities, including follicular development, embryo implantation and growth. The aim of this study was to evaluate LIF concentrations in serum and follicular fluid of women with PCOS and controls who underwent IVF with embryo transfer (IVF-ET). Serum and follicular fluid LIF concentrations were lower in women with PCOS compared with controls. Oestradiol concentrations in follicular fluid were higher in PCOS subjects compared with controls. LIF concentrations in serum (r = 0.6263, P < 0.05) and follicular fluid (r = 0.7093, P < 0.05) were negatively correlated with oestradiol concentration in the PCOS group. LIF concentrations in follicular fluid showed no difference between women who conceived and women who did not in both PCOS and control groups. However, LIF concentrations in embryo culture medium were higher in women who conceived following IVF compared with women who did not, in combined PCOS and control groups. The findings indicate that low LIF concentrations in serum and follicular fluid may contribute to disordered folliculogenesis in PCOS. LIF concentrations in embryo culture medium may predict the outcome of IVF treatment.
多囊卵巢综合征（PCOS）的特征是高雄激素血症、卵巢功能障碍和多囊卵巢形态。白血病抑制因子（LIF）影响许多生殖活动，包括卵泡发育、胚胎着床和生长。本研究旨在评估接受体外受精-胚胎移植（IVF-ET）的 PCOS 患者和对照组患者血清和卵泡液中的 LIF 浓度。与对照组相比，PCOS 患者的血清和卵泡液 LIF 浓度较低。与对照组相比，PCOS 患者的卵泡液中雌二醇浓度较高。PCOS 组中血清（r = 0.6263，P < 0.05）和卵泡液（r = 0.7093，P < 0.05）中的 LIF 浓度与雌二醇浓度呈负相关。在 PCOS 和对照组中，妊娠组和未妊娠组的卵泡液中 LIF 浓度均无差异。然而，在合并的 PCOS 和对照组中，与未妊娠的妇女相比，IVF 后妊娠的妇女的胚胎培养液中的 LIF 浓度更高。这些发现表明，血清和卵泡液中 LIF 浓度降低可能导致 PCOS 中卵泡生成障碍。胚胎培养液中的 LIF 浓度可能预测 IVF 治疗的结果。
