一种新的聚酮化合物紫生酸：通过硫酸二乙酯诱变海洋来源的产紫青霉G59激活聚酮化合物的生产。

A new polyketide purpurogenic acid: the activated production of polyketides by the diethyl sulphate mutagenesis of marine-derived Penicillium purpurogenum G59.

作者信息

Xia Ming-Wen, Yang Yu, Xu Rui, Li Chang-Wei, Cui Cheng-Bin

机构信息

a State Key Laboratory of Toxicology and Medical Countermeasures , Beijing Institute of Pharmacology and Toxicology , Beijing , China.

出版信息

Nat Prod Res. 2019 Jan;33(1):89-94. doi: 10.1080/14786419.2018.1434644. Epub 2018 Feb 4.

DOI:10.1080/14786419.2018.1434644

PMID:29397772

Abstract

A new polyketide, purpurogenic acid (1), and two known polyketides, (-)-mitorubrin (2) and (-)-mitorubrinol (3), were isolated from a fungal mutant derived from the diethyl sulphate (DES) mutagenesis of marine-derived Penicillium purpurogenum G59. The planar structure of new 1 was elucidated by spectroscopic methods and the absolute configuration was assigned on the basis of [α] and CD data. In our preliminary MTT assay, 1 inhibited human cancer K562, HL-60, HeLa and BGC-823 cells with the inhibition rates of 52.7, 78.8, 38.4 and 35.3% at the 100 μg/mL, respectively.

摘要

从海洋来源的产紫青霉G59经硫酸二乙酯（DES）诱变产生的真菌突变体中分离出一种新的聚酮化合物——产紫青霉酸（1）以及两种已知的聚酮化合物——（-）-米托蒽醌（2）和（-）-米托蒽醌醇（3）。通过光谱方法阐明了新化合物1的平面结构，并根据旋光和圆二色光谱数据确定了其绝对构型。在我们初步的MTT实验中，化合物1对人癌细胞K562、HL-60、HeLa和BGC-823具有抑制作用，在100μg/mL浓度下的抑制率分别为52.7%、78.8%、38.4%和35.3%。