Oui E, Habrioux G, Mathian B, Revol A, Henry R
Ann Biol Clin (Paris). 1986;44(1):58-62.
A sensitive and specific method for the determination of plasma S-DHA by gas chromatography (GC), with fused silica capillary column (stationary phase SE 54) and electron capture, after solvolysis of the sample and purification by high performance liquid chromatography (HPLC) is described. The interest of the new internal standard (5 alpha androst-9(11)-ene-3 beta ol-17-one) for the determination of the DHA by GC is shown. The analytical characteristics of this method as well as a comparative study of the values obtained by this method and by radioimmuno-assay are described.
描述了一种通过气相色谱法（GC）测定血浆S-DHA的灵敏且特异的方法，该方法采用熔融石英毛细管柱（固定相SE 54）和电子捕获法，样品先进行溶剂分解，然后通过高效液相色谱法（HPLC）纯化。展示了新内标物（5α-雄甾-9(11)-烯-3β-醇-17-酮）用于GC法测定DHA的优势。描述了该方法的分析特性以及对该方法与放射免疫分析法所得值的比较研究。
