Symes E K, Thomas B S
J Chromatogr. 1976 Jan 7;116(1):163-7. doi: 10.1016/s0021-9673(00)83711-5.
A method is described for the measurement of plasma dehydroepiandrosterone, as the iodomethyldimethylsilyl ether derivative, by gas-liquid chromatography and electron capture detection using the relatively new and highly stable stationary phase Dexsil 300. Preliminary purification of the plasma extract was required and alumina column chromatography was utilised, both before and after derivatization of the steroid extract. Specificity, precision, sensitivity and accuracy were all satisfactory. The method was used to study the relationship between age and the level of plasma dehydroepiandrosterone in a group of normal women. A significant negative correlation was observed.
本文描述了一种通过气液色谱法和电子捕获检测法，使用相对较新且高度稳定的固定相Dexsil 300来测量血浆脱氢表雄酮碘甲基二甲基硅醚衍生物的方法。血浆提取物需要进行初步纯化，在类固醇提取物衍生化之前和之后都采用了氧化铝柱色谱法。特异性、精密度、灵敏度和准确度均令人满意。该方法用于研究一组正常女性年龄与血浆脱氢表雄酮水平之间的关系。观察到显著的负相关。
