Jia Jianguang, Qin Yiyu, Zhang Ligong, Guo Chenxu, Wang Yaguo, Yue Xicheng, Qian Jun
Department of Oncology Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233003, P.R. China.
Department of Science and Technology, Jiading Central Hospital, Shanghai 201800, P.R. China.
Exp Ther Med. 2018 Feb;15(2):1718-1727. doi: 10.3892/etm.2017.5560. Epub 2017 Nov 24.
Sijunzi decoction (SJZD) is a traditional Chinese herbal medicine. Previous studies have indicated that SJZD exhibits antitumor activity. However, the underlying molecular mechanism has not been fully elucidated. To explore the antitumor mechanism of SJZD, the effects of serum from rats treated with SJZD on the proliferation of MKN-28 and HGC-27 gastric carcinoma cell lines were systematically investigated. It was found that SJZD-treated rat serum significantly inhibited the growth of MKN-28 and HGC-27 cells . The results obtained from a colony formation assay showed that SJZD-treated rat serum decreased the colony formation ability of MKN-28 and HGC-27 cells. The apoptosis rate in MKN-28 and HGC-27 cells was also increased following treatment with SJZD-treated rat serum. Flow cytometry with cell sorting revealed the presence of side population (SP) cells in MKN-28 and HGC-27 cells though Hoechst 33342 staining, and verapamil reduced the SP percentage. Further analysis showed that SJZD-treated rat serum promoted the apoptosis of SP cells in MKN-28 and HGC-27 cell lines by upregulating Bax, caspase-3 and PARP and downregulating bcl-2. These data revealed the therapeutic effect of SJZD-treated rat serum on gastric carcinoma. Following the preliminary identification of the inhibitory effect on the growth of gastric cancer cells , the growth inhibitory effect of SJZD-treated rat serum on SP cells was confirmed, and this inhibition particularly involved the induction of cell apoptosis.
四君子汤（SJZD）是一种传统中药。先前的研究表明，四君子汤具有抗肿瘤活性。然而，其潜在的分子机制尚未完全阐明。为了探究四君子汤的抗肿瘤机制，系统研究了用四君子汤处理的大鼠血清对MKN - 28和HGC - 27胃癌细胞系增殖的影响。发现用四君子汤处理的大鼠血清显著抑制MKN - 28和HGC - 27细胞的生长。集落形成试验结果表明，用四君子汤处理的大鼠血清降低了MKN - 28和HGC - 27细胞的集落形成能力。用四君子汤处理的大鼠血清处理后，MKN - 28和HGC - 27细胞的凋亡率也增加。通过细胞分选的流式细胞术通过Hoechst 33342染色揭示了MKN - 28和HGC - 27细胞中侧群（SP）细胞的存在，维拉帕米降低了SP百分比。进一步分析表明，用四君子汤处理的大鼠血清通过上调Bax、caspase - 3和PARP并下调bcl - 2促进了MKN - 28和HGC - 27细胞系中SP细胞的凋亡。这些数据揭示了用四君子汤处理的大鼠血清对胃癌的治疗作用。在初步鉴定对胃癌细胞生长的抑制作用后，证实了用四君子汤处理的大鼠血清对SP细胞的生长抑制作用，并且这种抑制尤其涉及细胞凋亡的诱导。
