Zhang Meng, Xiong Dedong, Li Hongxia, Wang Zonglan, Li Renzhe
Department of Clinical Laboratory, Binzhou City Center Hospital, Binzhou, Shandong 251700, P.R. China.
Department of Obstetrics, Binzhou City Center Hospital, Binzhou, Shandong 251700, P.R. China.
Exp Ther Med. 2018 Feb;15(2):1871-1874. doi: 10.3892/etm.2017.5464. Epub 2017 Nov 8.
The purpose of the present study was to investigate the diagnostic value of T cells spot test (T-Spot TB) combined with interferon-γ (INF-γ) and interleukin-27 (IL-27) in tuberculous pleurisy. Sixty patients with tuberculous pleurisy (observation group) and 60 patients with non-tuberculous pleurisy (control group) were enrolled in this study. T-Spot TB was performed to detect the pleural effusion of two groups of patients. Levels of IFN-γ and IL-27 in serum and pleural effusion were detected by enzyme-linked immunosorbent assay (ELISA). Relative expression of IFN-γ mRNA and IL-27 mRNA in peripheral blood mononuclear cells were detected by RT-PCR. Positive rate of T-Spot TB in observation group was 96.7% (58 cases), which was significantly higher than that in control group (p<0.05). Concentration of INF-γ in pleural effusion of observation group was 468.6±24.8 ng/l, which was significantly higher than that in control group (131.3±18.7 ng/l, p<0.05). Concentration of IL-27 in pleural effusion of observation group was 423.4±37.2 ng/l, which was significantly higher than that in control group (116.2±15.5 ng/l, p<0.05). Concentrations of INF-γ and IL-27 in serum of observation group were 48.2±13.4 and 41.7±10.6 ng/l, respectively, which were significantly higher than those in control group (38.6±11.2 and 35.3±8.4 ng/l, p<0.05). Relative expression levels of INF-γ mRNA and IL-27 mRNA in observation group were significantly higher than those in control group (p<0.05). Therefore, combination of T-Spot TB with INF-γ and IL-27 has significant application value in the clinical diagnosis of tuberculous pleurisy, and should be popularized.
本研究旨在探讨T细胞斑点试验（T-Spot TB）联合干扰素-γ（INF-γ）和白细胞介素-27（IL-27）在结核性胸膜炎中的诊断价值。本研究纳入了60例结核性胸膜炎患者（观察组）和60例非结核性胸膜炎患者（对照组）。对两组患者的胸腔积液进行T-Spot TB检测。采用酶联免疫吸附测定（ELISA）法检测血清和胸腔积液中INF-γ和IL-27的水平。采用逆转录聚合酶链反应（RT-PCR）检测外周血单个核细胞中INF-γ mRNA和IL-27 mRNA的相对表达。观察组T-Spot TB阳性率为96.7%（58例），显著高于对照组（p<0.05）。观察组胸腔积液中INF-γ浓度为468.6±24.8 ng/l，显著高于对照组（131.3±18.7 ng/l，p<0.05）。观察组胸腔积液中IL-27浓度为423.4±37.2 ng/l，显著高于对照组（116.2±15.5 ng/l，p<0.05）。观察组血清中INF-γ和IL-27浓度分别为48.2±13.4和41.7±10.6 ng/l，显著高于对照组（38.6±11.2和35.3±8.4 ng/l，p<0.05）。观察组INF-γ mRNA和IL-27 mRNA的相对表达水平显著高于对照组（p<0.05）。因此，T-Spot TB联合INF-γ和IL-27在结核性胸膜炎的临床诊断中具有显著的应用价值，值得推广。
