Li Diandian, Shen Yongchun, Fu Xiaomin, Li Min, Wang Tao, Wen Fuqiang
Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu 610041, China ; Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, Sichuan University Chengdu 610000, China.
Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu 610041, China.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):888-93. eCollection 2015.
To investigate the diagnostic accuracy of the combination of interleukin-33 (IL-33) and adenosine deaminase (ADA) for differentiating TPE from pleural effusions with the other etiologies.
Pleural effusion samples were collected from 32 TPE patients and 55 non-TPE patients. Pleural levels of IL-33 and ADA were measured by ELISA. The corresponding biochemical indexes were also simultaneously determined.
The pleural levels of IL-33 and ADA in the TPE group were significantly higher than those in the non-TPE group. With a cut-off value of 68.3 pg/ml, the sensitivity and specificity for IL-33 were 83.9% and 70.9%, respectively. While for ADA, the sensitivity and specificity were 87.5% and 87.3%, respectively at a cut-off value of 10.25 U/L. Combined use of IL-33 and ADA measurements further increased the sensitivity or specificity.
Our study suggests that the applications of new biomarker IL-33, along with ADA, may serve as efficient diagnosis strategies in the management of pleural TB. Further studies at a large scale should be performed to validate our findings.
探讨白细胞介素-33（IL-33）与腺苷脱氨酶（ADA）联合检测对结核性胸膜炎（TPE）与其他病因所致胸腔积液的诊断准确性。
收集32例TPE患者和55例非TPE患者的胸腔积液样本。采用酶联免疫吸附测定法（ELISA）检测胸腔积液中IL-33和ADA水平。同时测定相应的生化指标。
TPE组胸腔积液中IL-33和ADA水平显著高于非TPE组。以68.3 pg/ml为临界值，IL-33的敏感性和特异性分别为83.9%和70.9%。而对于ADA，以10.25 U/L为临界值时，敏感性和特异性分别为87.5%和87.3%。联合检测IL-33和ADA可进一步提高敏感性或特异性。
我们的研究表明，新的生物标志物IL-33与ADA联合应用，可能是结核性胸膜炎诊断的有效策略。应开展大规模进一步研究以验证我们的发现。
