Department of BioScience and Technology for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini 1, Teramo 64100, Italy.
Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, I-40127 Bologna, Italy.
FEMS Microbiol Lett. 2018 Mar 1;365(6). doi: 10.1093/femsle/fny028.
In this study, 29 strains of Kluyveromyces marxianus with peculiar genetic and phenotypic traits previously isolated from a fermented goat milk of Yaghnob valley were investigated for chromosome length polymorphism (CLP) by PFGE, adhesion properties and carbon usage by Biolog analysis. Obtained data showed that strains differed in terms of number and size of chromosome bands. The number of bands ranged from 5 to 7, suggesting a probable genome size from 1.4 to 2.6 Mb. Strains showed a certain level of cell surface hydrophobicity ranging from 32% to 77.7%. Strains were also tested for their ability to form a biofilm on polystyrene plates: planktonic cells ranged from 6.3 cfu/mL to 7.95 cfu/mL, while sessile from 7.11 cfu/mL to 8.6 cfu/mL. The strains able to adhere to polystyrene plates were also able to form a mature MAT. Biolog analysis revealed that almost all strains were able to use putrescine, malic acid, α-D lactose, phenylethylamine, β-methyl D-gucoside and xylose; 5 strains were able to grow on cellobiose and 3 were able to catabolise α-ketobutyric. The obtained data highlighted a number of interesting features underlying the peculiar capacities of these strains for industrial applications.
在这项研究中，对 29 株来自亚格诺山谷发酵羊奶的具有特殊遗传和表型特征的马克斯克鲁维酵母 Kluyveromyces marxianus 菌株进行了 PFGE 染色体长度多态性（CLP）、粘附特性和 Biolog 分析碳利用的研究。获得的数据表明，菌株在染色体带的数量和大小方面存在差异。带的数量范围从 5 到 7，表明可能的基因组大小从 1.4 到 2.6 Mb。菌株表现出一定程度的细胞表面疏水性，范围从 32%到 77.7%。还测试了菌株在聚苯乙烯平板上形成生物膜的能力：浮游细胞范围从 6.3 cfu/mL 到 7.95 cfu/mL，而固定细胞范围从 7.11 cfu/mL 到 8.6 cfu/mL。能够粘附聚苯乙烯平板的菌株也能够形成成熟的 MAT。Biolog 分析表明，几乎所有菌株都能够利用腐胺、苹果酸、α-D 乳糖、苯乙胺、β-甲基 D-吡喃葡萄糖苷和木糖；5 株菌株能够在纤维二糖上生长，3 株菌株能够分解α-酮丁酸。所得数据突出了这些菌株具有工业应用的特殊能力所具有的一些有趣特征。
