Abu-Sinna E, Hasan M Y, El-Deftar M M, Amer S A, Abdelsalam L O, Nakhla J A
Department of Pathology, National Cancer Institute, Cairo University, Faculty of Medicine, Cairo University, Egypt.
Department of Pathology, Faculty of Medicine, Cairo University, Egypt.
J Cytol. 2018 Jan-Mar;35(1):27-32. doi: 10.4103/JOC.JOC_67_17.
To test the expression of galectin-3 (gal-3) and Hector Battifora mesothelial antigen-1 (HBME-1) on agarose cell blocks (CBs) of fine-needle aspirates aiming to determine their diagnostic value in thyroid follicle cell-derived tumors.
Forty patients with thyroid nodule were enrolled. Fine-needle aspiration biopsy was done and processed to produce smears and CBs. Immune staining was done on CBs using antibodies to gal-3 and HBME-1. Diagnostic value of tests was determined in comparison to pathology.
Current study included 17 papillary thyroid carcinoma (PTC), 15 follicular adenoma, and 8 follicular thyroid carcinoma (FTC). In PTC diagnosis, co-expression of gal-3/HBME-1 had a sensitivity of 94.1%, specificity of 73.3%, positive predictive value (PPV) of 80%, negative predictive value (NPV) 91.7%, and accuracy of 84.4%. In FTC diagnosis, combined gal-3/HBME-1 expression had a sensitivity of 75%, specificity of 78.6%, PPV of 50%, NPV of 91.7%, and accuracy of 77.8%. Co-expression of gal-3/HBME-1 raised the sensitivity of detection of differentiated thyroid carcinomas from 40% with cytomorphology to 92% and accuracy from 62.5% to 85%.
The combined immunocytochemical expression of gal-3 and HBME-1 utilizing fine-needle aspirates can improve the sensitivity of detection and diagnostic accuracy of differentiated follicular cell-derived thyroid carcinomas.
检测细针穿刺抽吸物的琼脂糖细胞块（CBs）上半乳糖凝集素-3（gal-3）和赫克托·巴蒂福拉间皮抗原-1（HBME-1）的表达，旨在确定其在甲状腺滤泡细胞源性肿瘤中的诊断价值。
纳入40例甲状腺结节患者。进行细针穿刺活检并制作涂片和细胞块。使用抗gal-3和HBME-1抗体对细胞块进行免疫染色。与病理结果比较确定检测的诊断价值。
本研究包括17例乳头状甲状腺癌（PTC）、15例滤泡性腺瘤和8例滤泡状甲状腺癌（FTC）。在PTC诊断中，gal-3/HBME-1的共表达敏感性为94.1%，特异性为73.3%，阳性预测值（PPV）为80%，阴性预测值（NPV）为91.7%，准确性为84.4%。在FTC诊断中，gal-3/HBME-1联合表达敏感性为75%，特异性为78.6%，PPV为50%，NPV为91.7%，准确性为77.8%。gal-3/HBME-1的共表达将分化型甲状腺癌的检测敏感性从细胞形态学的40%提高到92%，准确性从62.5%提高到85%。
利用细针穿刺抽吸物联合检测gal-3和HBME-1的免疫细胞化学表达可提高分化型滤泡细胞源性甲状腺癌的检测敏感性和诊断准确性。
