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大黄附子汤通过调节肠黏膜上皮细胞线粒体通透性转换孔减轻重症急性胰腺炎相关肠损伤。

Dai-Huang-Fu-Zi-Tang Alleviates Intestinal Injury Associated with Severe Acute Pancreatitis by Regulating Mitochondrial Permeability Transition Pore of Intestinal Mucosa Epithelial Cells.

作者信息

Kang Xin, Liang Zhengkai, Lu Xiaoguang, Zhan Libin, Song Jianbo, Wang Yi, Yang Yilun, Fan Zhiwei, Bai Lizhi

机构信息

Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian 116001, China.

Gastrointestinal Surgery, Liaocheng City People's Hospital, Liaocheng 252000, China.

出版信息

Evid Based Complement Alternat Med. 2017;2017:4389048. doi: 10.1155/2017/4389048. Epub 2017 Dec 18.

DOI:10.1155/2017/4389048
PMID:29403537
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5748303/
Abstract

OBJECTIVE

The aim of the present study was to examine whether Dai-Huang-Fu-Zi-Tang (DHFZT) could regulate mitochondrial permeability transition pore (MPTP) of intestinal mucosa epithelial cells for alleviating intestinal injury associated with severe acute pancreatitis (SAP).

METHODS

A total of 72 Sprague-Dawley rats were randomly divided into 3 groups (sham group, SAP group, and DHFZT group, = 24 per group). The rats in each group were divided into 4 subgroups ( = 6 per subgroup) accordingly at 1, 3, 6, and 12 h after the operation. The contents of serum amylase, D-lactic acid, diamine oxidase activity, and degree of MPTP were measured by dry chemical method and enzyme-linked immunosorbent assay. The change of mitochondria of intestinal epithelial cells was observed by transmission electron microscopy.

RESULTS

The present study showed that DHFZT inhibited the openness of MPTP at 3, 6, and 12 h after the operation. Meanwhile, it reduced the contents of serum D-lactic acid and activity of diamine oxidase activity and also drastically relieved histopathological manifestations and epithelial cells injury of intestine.

CONCLUSION

DHFZT alleviates intestinal injury associated SAP via reducing the openness of MPTP. In addition, DHFZT could also decrease the content of serum diamine oxidase activity and D-lactic acid after SAP.

摘要

目的

本研究旨在探讨大黄附子汤（DHFZT）是否能调节肠黏膜上皮细胞线粒体通透性转换孔（MPTP），以减轻重症急性胰腺炎（SAP）相关的肠损伤。

方法

将72只Sprague-Dawley大鼠随机分为3组（假手术组、SAP组和DHFZT组，每组24只）。每组大鼠在术后1、3、6和12小时相应地分为4个亚组（每个亚组6只）。采用干化学法和酶联免疫吸附测定法检测血清淀粉酶、D-乳酸、二胺氧化酶活性及MPTP程度。通过透射电子显微镜观察肠上皮细胞线粒体的变化。

结果

本研究表明，DHFZT在术后3、6和12小时抑制了MPTP的开放。同时，它降低了血清D-乳酸含量和二胺氧化酶活性，也显著减轻了肠道的组织病理学表现和上皮细胞损伤。

结论

DHFZT通过降低MPTP的开放程度减轻与SAP相关的肠损伤。此外，DHFZT还可降低SAP后血清二胺氧化酶活性和D-乳酸含量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cab/5748303/eea67e44d774/ECAM2017-4389048.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cab/5748303/01a7a3f1e3b2/ECAM2017-4389048.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cab/5748303/bdb0259940ff/ECAM2017-4389048.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cab/5748303/784c8f1a16f8/ECAM2017-4389048.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cab/5748303/eea67e44d774/ECAM2017-4389048.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cab/5748303/01a7a3f1e3b2/ECAM2017-4389048.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cab/5748303/bdb0259940ff/ECAM2017-4389048.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cab/5748303/784c8f1a16f8/ECAM2017-4389048.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0cab/5748303/eea67e44d774/ECAM2017-4389048.004.jpg

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