Lu Xiaoguang, Kang Xin, Zhan Libin, Lv Chunyu, Fan Zhiwei, Wang Yingli, Ali Robbie, Lv Chang, Li Siyao, Mu Jinhai
Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian 116001, China.
BMC Complement Altern Med. 2014 Mar 1;14:80. doi: 10.1186/1472-6882-14-80.
Dai Huang Fu Zi Tang (DHFZT), an oriental herbal formula, has long been used clinically in treatment of intestinal obstruction, acute pancreatitis, cholecystalgia and chronic diarrhea for thousands of years. Recent studies have demonstrated that DHFZT can reduce intestinal pathological injury and the concentration of enterogenous endotoxin in an animal model. But the underlying mechanism has not been fully elucidated.
SD male rats in adult were used to model HS and treated with DHFZT. The serum concentration of endotoxin were analyzed by dynamic turbidimetric method. In addition, the blood flow of small intestine were measured using laser speckle technique. Phosphorylated vasodilator-stimulated phosphoprotein (p-VASP) and zonula occludens (ZO)-1 protein, intestinal fatty acid binding protein (IFABP) were measured by Western Blotting, RT-PCR, ELISA respectively.
Present study showed that DHFZT markedly elevated the blood flow of small intestine, protected the intestinal barrier function by up-regulating the expression of ZO-1 protein and down-regulating expression of p-VASP, and notely decreased serum concentration of IFABP and endotoxin in HS.
These results reveal that DHFZT improves intestinal blood flow, protects the intestinal barrier function, and ameliorates intestinal endotoxaemia in rats with HS.
大黄附子汤（DHFZT）是一种中药方剂，数千年来一直被临床用于治疗肠梗阻、急性胰腺炎、胆囊炎和慢性腹泻。最近的研究表明，在动物模型中，大黄附子汤可以减轻肠道病理损伤并降低肠源性内毒素的浓度。但其潜在机制尚未完全阐明。
选用成年雄性SD大鼠建立热射病（HS）模型，并用大黄附子汤进行治疗。采用动态浊度法分析血清内毒素浓度。此外，使用激光散斑技术测量小肠血流量。分别通过蛋白质免疫印迹法、逆转录聚合酶链反应、酶联免疫吸附测定法检测磷酸化血管舒张刺激磷蛋白（p-VASP）、紧密连接蛋白（ZO）-1、肠脂肪酸结合蛋白（IFABP）。
本研究表明，大黄附子汤可显著提高小肠血流量，通过上调ZO-1蛋白表达和下调p-VASP表达来保护肠道屏障功能，并显著降低热射病大鼠血清中IFABP和内毒素的浓度。
这些结果表明，大黄附子汤可改善热射病大鼠的肠道血流量，保护肠道屏障功能，并改善肠道内毒素血症。
