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使用超声联合微泡将神经营养因子-3转染至神经干细胞以治疗失神经肌肉萎缩。

Transfection of neurotrophin-3 into neural stem cells using ultrasound with microbubbles to treat denervated muscle atrophy.

作者信息

Gong Lin, Jiang Changqing, Liu Li, Wan Shengxiang, Tan Wen, Ma Sushuang, Jia Xiaojian, Wang Meiwei, Hu Azhen, Shi Yu, Zhang Yu, Shen Yuanyuan, Wang Feng, Chen Yun

机构信息

Department of Ultrasound, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.

Department of Sports Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.

出版信息

Exp Ther Med. 2018 Jan;15(1):620-626. doi: 10.3892/etm.2017.5439. Epub 2017 Nov 6.

DOI:10.3892/etm.2017.5439
PMID:29403547
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5780738/
Abstract

Neurotrophin-3 (NT-3) has potential as a therapeutic agent for the treatment of patients with denervated muscle atrophy. However, the endogenous secretion of NT-3 is low and exogenous NT-3 lacks sufficient time to accumulate due to its short half-life. The transfection of NT-3 has been demonstrated to have a beneficial effect on denervated muscle and motor endplates. Neural stem cells (NSCs) differentiate into neurons and form motor endplate nerve-muscle connections. It has been previously demonstrated that local and noninvasive transfection can be performed using ultrasound with microbubbles (MBs). In the current study, hematoxylin and eosin, acetylcholinesterase and gold chloride staining, as well as transmission electron microscopy, were performed to verify the effects of this treatment strategy. The results demonstrated that using ultrasound with MBs for the transfection of NT-3 into NSCs, and their subsequent transplantation , attenuated the atrophy of denervated muscle and reduced motor endplate degeneration. This noninvasive, efficient and targeted treatment strategy may therefore be a potential treatment for patients with denervated muscle atrophy.

摘要

神经营养因子-3（NT-3）具有作为治疗失神经肌肉萎缩患者的治疗剂的潜力。然而，NT-3的内源性分泌较低，并且外源性NT-3由于其半衰期短而缺乏足够的时间来积累。NT-3的转染已被证明对失神经肌肉和运动终板有有益作用。神经干细胞（NSCs）分化为神经元并形成运动终板神经-肌肉连接。先前已经证明可以使用带有微泡（MBs）的超声进行局部和非侵入性转染。在本研究中，进行苏木精和伊红染色、乙酰胆碱酯酶和氯化金染色以及透射电子显微镜检查以验证该治疗策略的效果。结果表明，使用带有MBs的超声将NT-3转染到NSCs中，以及随后进行移植，减轻了失神经肌肉的萎缩并减少了运动终板退变。因此，这种非侵入性、高效且靶向的治疗策略可能是失神经肌肉萎缩患者的一种潜在治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/8a25d1cdbd52/etm-15-01-0620-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/df3326458fc9/etm-15-01-0620-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/29254bd52a2e/etm-15-01-0620-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/a76660ca281d/etm-15-01-0620-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/872cd5ac35e8/etm-15-01-0620-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/76fe5b5e6fdb/etm-15-01-0620-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/7cc383127ac2/etm-15-01-0620-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/d061c2d580a0/etm-15-01-0620-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/8a25d1cdbd52/etm-15-01-0620-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/df3326458fc9/etm-15-01-0620-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/29254bd52a2e/etm-15-01-0620-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/a76660ca281d/etm-15-01-0620-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/872cd5ac35e8/etm-15-01-0620-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/76fe5b5e6fdb/etm-15-01-0620-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/7cc383127ac2/etm-15-01-0620-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/d061c2d580a0/etm-15-01-0620-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d3/5780738/8a25d1cdbd52/etm-15-01-0620-g07.jpg

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J Appl Physiol (1985). 2016 Feb 15;120(4):426-36. doi: 10.1152/japplphysiol.00175.2015. Epub 2015 Dec 17.
2
A novel UCS memory retrieval-extinction procedure to inhibit relapse to drug seeking.一种抑制药物寻求复发的新型UCS记忆检索-消退程序。
Nat Commun. 2015 Jul 14;6:7675. doi: 10.1038/ncomms8675.
3
Mechanisms of microbubble-facilitated sonoporation for drug and gene delivery.
超声辐射及 SonoVue 微泡介导的 PRR11 靶向 RNA 干扰抑制乳腺癌细胞增殖和转移，但促进细胞凋亡。
Biosci Rep. 2020 Nov 27;40(11). doi: 10.1042/BSR20201854.
4
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Onco Targets Ther. 2019 Aug 21;12:6813-6824. doi: 10.2147/OTT.S209692. eCollection 2019.
微泡辅助声孔效应用于药物和基因递送的机制。
Ther Deliv. 2014 Apr;5(4):467-86. doi: 10.4155/tde.14.10.
4
Flow-focusing regimes for accelerated production of monodisperse drug-loadable microbubbles toward clinical-scale applications.流聚焦状态可加速生产适用于临床应用的载药单分散微泡。
Lab Chip. 2013 Dec 21;13(24):4816-26. doi: 10.1039/c3lc51016f.
5
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7
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