Nomikou Nikolitsa, Feichtinger Georg A, Redl Heinz, McHale Anthony P
Sonidel Ltd, Dublin, Ireland.
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Austrian Cluster for Tissue Regeneration, Vienna, Austria.
J Tissue Eng Regen Med. 2016 Jan;10(1):29-39. doi: 10.1002/term.1730. Epub 2013 Apr 17.
It has been suggested that gene transfer into donor cells is an efficient and practical means of locally supplying requisite growth factors for applications in tissue regeneration. Here we describe, for the first time, an ultrasound-mediated system that can non-invasively facilitate gene transfer into cells entrapped within fibrin-based matrices. Since ultrasound-mediated gene transfer is enhanced using microbubbles, we compared the efficacy of neutral and cationic forms of these reagents on the ultrasound-stimulated gene transfer process in gel matrices. In doing so we demonstrated the beneficial effects associated with the use of cationic microbubble preparations that interact directly with cells and nucleic acid within matrices. In some cases, gene expression was increased two-fold in gel matrices when cationic microbubbles were compared with neutral microbubbles. In addition, incorporating collagen into fibrin gels yielded a 25-fold increase in gene expression after application of ultrasound to microbubble-containing matrices. We suggest that this novel system may facilitate non-invasive temporal and spatial control of gene transfer in gel-based matrices for the purposes of tissue regeneration.
有人提出，将基因导入供体细胞是一种有效且实用的方法，可在组织再生应用中局部提供所需的生长因子。在此，我们首次描述了一种超声介导的系统，该系统能够以非侵入性方式促进基因导入包裹在纤维蛋白基基质中的细胞。由于使用微泡可增强超声介导的基因转移，我们比较了这些试剂的中性和阳离子形式对凝胶基质中超声刺激的基因转移过程的功效。在此过程中，我们证明了使用与基质中的细胞和核酸直接相互作用的阳离子微泡制剂的有益效果。在某些情况下，与中性微泡相比，阳离子微泡在凝胶基质中的基因表达增加了两倍。此外，将胶原蛋白掺入纤维蛋白凝胶中，在对含微泡的基质施加超声后，基因表达增加了25倍。我们认为，这种新型系统可能有助于在基于凝胶的基质中对基因转移进行非侵入性的时空控制，以实现组织再生的目的。
