Pegha-Moukandja Irène, Imboumy-Limoukou Roméo-Karl, Tchitoula-Makaya Nina, Mouinga-Ondeme Augustin-Ghislain, Biteghe-Bi-Essone Jean Claude, Mba Dieudonne Nkoghe, Lekana-Douki Jean-Bernard, Ndouo Fousseyni S Toure
Centre International de Recherches Médicales de Franceville (CIRMF), BP 769, Franceville Gabon, Africa.
Département de Parasitologie-Mycologie et Médecine Tropicale, Faculté de Médecine, Université des Sciences de la Santé, BP 4009, Libreville, Gabon, Africa.
Eur J Microbiol Immunol (Bp). 2017 Dec 4;7(4):247-260. doi: 10.1556/1886.2017.00010. eCollection 2017 Dec 18.
merozoite antigens (PfMAgs) play an essential role in the development of immunity to malaria. Currently, protein 113 (Pf 113), apical membrane antigen 1 (AMA1), erythrocyte binding antigens (EBA175), and reticulocyte binding protein homologue 5 (RH5) are among the most PfMAgs studied. A comparative analysis of naturally acquired antibodies against these antigens in children would increase our knowledge about the development of protective immunity. Analysis of antibodies to Pf113, PfAMA1, PfEBA175, and PfRH5 was conducted in rural population during 2013 and 2014. Both prevalence and levels of total IgG anti-PfAMA1 were higher than that of IgG anti-PfEBA175, anti-PfRH5, and anti-Pf113. Seroconversion to PfAMA1 and PfEBA175 occurred moderately in young children and reached to the maximum in adolescent and in adults. High prevalence of IgG anti-Pf113 was observed in young children of 3 to 6 years old in 2013. The four antigens were recognized by IgG 1, 2, 3, and 4 antibodies from a large proportion of the subjects, and all of them induced high levels of specific IgG1 against PfAMA1, PfEBA175, fewer by Pf113 and PfRH5. Many asymptomatic children had specific IgG1 recognizing multiple antigens, and these IgG1 antibodies could be associated with a reduced risk of developing malaria symptoms.
裂殖子抗原（PfMAgs）在疟疾免疫发展中起关键作用。目前，蛋白113（Pf 113）、顶膜抗原1（AMA1）、红细胞结合抗原（EBA175）和网织红细胞结合蛋白同源物5（RH5）是研究最多的PfMAgs。对儿童中针对这些抗原的自然获得性抗体进行比较分析，将增加我们对保护性免疫发展的了解。2013年至2014年期间，对农村人口中针对Pf113、PfAMA1、PfEBA175和PfRH5的抗体进行了分析。抗PfAMA1的总IgG流行率和水平均高于抗PfEBA175、抗PfRH5和抗Pf113。幼儿中向PfAMA1和PfEBA175的血清转化适度发生，并在青少年和成年人中达到最高。2013年，在3至6岁的幼儿中观察到抗Pf113的高流行率。很大一部分受试者的IgG 1、2、3和4抗体识别这四种抗原，并且所有这些抗体都诱导产生了高水平的针对PfAMA1、PfEBA175的特异性IgG1，针对Pf113和PfRH5的较少。许多无症状儿童具有识别多种抗原的特异性IgG1，并且这些IgG1抗体可能与疟疾症状发生风险降低有关。
