伏安法测定生物基质和药物制剂中抗肝炎药物阿德福韦的含量。
Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation.
作者信息
Jain Rajeev, Sharma Ramkishor
机构信息
School of Studies in Chemistry, Jiwaji University, Gwalior 474011, India.
出版信息
J Pharm Anal. 2012 Apr;2(2):98-104. doi: 10.1016/j.jpha.2011.10.002. Epub 2011 Nov 20.
Electrochemical reduction behavior of Adefovir was studied using Hanging Mercury Drop Electrode (HMDE) in Britton-Robinson (BR) buffer solution. Voltammetric study showed one well-defined reduction peak in the potential range -1.2 to -1.4 V (vs. Ag/AgCl) due to reduction of C=N bond of the imidazole ring. Solid-phase extraction and protein-precipitation techniques were employed for extraction of Adefovir from human plasma. The proposed method allows quantification of Adefovir in human plasma over the concentration range 0.50-5.00 μg/mL with the detection limit 0.17 μg/mL, whereas in pharmaceutical formulation 0.25-2.25 μg/mL with the detection limit 0.08 μg/mL.
采用悬汞滴电极（HMDE）在 Britton-Robinson（BR）缓冲溶液中研究了阿德福韦的电化学还原行为。伏安法研究表明，由于咪唑环的 C=N 键还原，在 -1.2 至 -1.4 V（相对于 Ag/AgCl）的电位范围内出现一个明确的还原峰。采用固相萃取和蛋白质沉淀技术从人血浆中提取阿德福韦。所提出的方法能够定量测定人血浆中浓度范围为 0.50 - 5.00 μg/mL 的阿德福韦，检测限为 0.17 μg/mL，而在药物制剂中浓度范围为 0.25 - 2.25 μg/mL，检测限为 0.08 μg/mL。
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