Jain Rajeev, Sharma Ramkishor
School of Studies in Chemistry, Jiwaji University, Gwalior 474011, India.
J Pharm Anal. 2012 Dec;2(6):443-449. doi: 10.1016/j.jpha.2012.03.005. Epub 2012 Mar 18.
Granisetron showed one well-defined reduction peak at Hanging Mercury Drop Electrode (HMDE) in the potential range from -1.3 to -1.5 V due to reduction of C=N bond. Solid-phase extraction technique was employed for extraction of Granisetron from spiked human plasma. Granisetron showed peak current enhancement of 4.45% at square-wave voltammetry and 5.33% at cyclic voltammetry as compared with the non stripping techniques. The proposed voltammetric method allowed quantification of Granisetron in pharmaceutical formulation over the target concentration range of 50-200 ng/mL with detection limit 13.63 ng/mL, whereas in human plasma 50-225 ng/mL with detection limit 11.75 ng/mL.
由于C=N键的还原，格拉司琼在悬汞滴电极（HMDE）上于-1.3至-1.5 V的电位范围内显示出一个明确的还原峰。采用固相萃取技术从加标的人血浆中萃取格拉司琼。与非溶出技术相比，格拉司琼在方波伏安法中显示出4.45%的峰电流增强，在循环伏安法中显示出5.33%的峰电流增强。所提出的伏安法能够在50 - 200 ng/mL的目标浓度范围内对药物制剂中的格拉司琼进行定量，检测限为13.63 ng/mL，而在人血浆中为50 - 225 ng/mL，检测限为11.75 ng/mL。
