Wu Ai-Zhi, Lin Chao-Zhan, Zhai Ya-Jing, Zhuo Jia-Lin, Zhu Chen-Chen
School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
J Pharm Anal. 2013 Feb;3(1):61-65. doi: 10.1016/j.jpha.2012.07.001. Epub 2012 Jul 8.
Two phenylethanoid glycosides, acteoside and forsythoside B, were first isolated from the traditional Chinese herb H.T. Chang. The interaction between the two phenylethanoid glycosides and bovine serum albumin (BSA) was investigated by fluorescence, UV-vis absorbance and circular dichroism (CD). The results showed that the quenching mechanism in the drug-BSA binary systems was a combination of static quenching and non-radiative energy transfer. Displacement experiments confirmed that the drug bound to the site I of BSA. UV-vis and CD measurements indicated that the binding of the drug to BSA induced conformational changes in BSA.
两种苯乙醇苷类化合物，毛蕊花糖苷和连翘酯苷B，首次从传统中药长花胡颓子中分离得到。通过荧光、紫外可见吸收光谱和圆二色光谱（CD）研究了这两种苯乙醇苷类化合物与牛血清白蛋白（BSA）之间的相互作用。结果表明，药物 - BSA二元体系中的猝灭机制是静态猝灭和非辐射能量转移的结合。置换实验证实药物与BSA的位点I结合。紫外可见光谱和CD测量表明药物与BSA的结合诱导了BSA的构象变化。
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