Suleiman Suleiman Ahmed, Song Fan, Su Mengxiang, Hang Taijun, Song Min
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.
J Pharm Anal. 2017 Feb;7(1):48-55. doi: 10.1016/j.jpha.2016.06.001. Epub 2016 Jun 21.
A suitable liquid chromatography quadrupole time-of-flight mass spectrometric (LC-Q-TOF-MS) method was developed for separation and characterization of related substances in bacitracin test drug. The separation was performed on LiChrospher RP-18 column using methanol as mobile phase A and 0.2% ammonium acetate buffer solution as mobile phase B in gradient elution. A total of 12 related substances were detected through high resolution mass spectrometric determination in a positive electrospray ionization mode. They were identified as co-existing active components and degradation products of bacitracin through the analysis and elucidation of both the protonated parents and the product ions of all the related substances and their fragmentation pathways were also proposed.
建立了一种合适的液相色谱-四极杆飞行时间质谱（LC-Q-TOF-MS）方法，用于分离和表征杆菌肽受试药物中的有关物质。采用LiChrospher RP-18柱进行分离，以甲醇为流动相A，0.2%醋酸铵缓冲溶液为流动相B进行梯度洗脱。在正电喷雾电离模式下，通过高分辨率质谱测定共检测到12种有关物质。通过对所有有关物质的质子化母离子和产物离子的分析和解析，将它们鉴定为杆菌肽共存的活性成分和降解产物，并提出了它们的碎裂途径。
