Sobol R E, Mick R, LeBien T W, Ozer H, Minowada J, Anderson K, Ellison R R, Cuttner J, Morrison A, Richards F
Leuk Res. 1986;10(5):481-5. doi: 10.1016/0145-2126(86)90083-4.
Peripheral blood and/or bone marrow lymphoblasts from 25 patients with acute lymphoblastic leukemia (ALL) were tested with a panel of monoclonal antibodies (MoAbs) and conventional hematopoietic markers by three different laboratories. The results were analysed to evaluate the reproducibility of ALL phenotype determinations. Specimens were transported between laboratories by 24-h courier service and were classified on the basis of indirect immunofluorescence MoAb reactivities as follows: B-lineage ALL (BA-1+T-MCS-2-); T-lineage ALL (T+BA-1-MCS-2-); myeloid antigen ALL (MCS-2+BA-1-CALLA-T-) and unclassified ALL (BA-1-MCS-2-CALLA-T-). Conventional marker studies for surface immunoglobulin (sIg), cytoplasmic immunoglobulin (cIg), sheep erythrocyte rosette formation (E) and nuclear terminal deoxynucleotidyl transferase (TdT) were also performed. In the cases with sufficient marker data to permit classification, 90% (18/20) were identically classified by different laboratories and this concordance was statistically significant (p less than 0.05). The agreement between laboratories for individual MoAb and conventional marker analyses was statistically significant (p less than 0.05) for all markers with the exception of BA-2, cIg and TdT determinations. Six of 7 discordant BA-2 cases represented BA-2+ evaluations which had subsequent BA-2- results following specimen transportation. These findings suggest instability of the BA-2 antigen to transport conditions. A similar pattern of positive to negative evaluations following transportation was observed in 5/5 discordant results involving other MoAbs. Disagreement between laboratories for cIg and TdT determinations implies that the detection of cytoplasmic or nuclear antigens may be more prone to subjective interpretation than cell surface antigen marker analyses. Our findings suggest that immunofluorescence marker studies employing MoAbs to cell surface antigens are in general highly reproducible. Our results also indicate that specimen storage conditions and the cellular location of target antigens are important variables which may affect the reproducibility of ALL phenotype determinations.
来自25例急性淋巴细胞白血病（ALL）患者的外周血和/或骨髓淋巴母细胞由三个不同实验室用一组单克隆抗体（MoAbs）和传统造血标志物进行检测。分析结果以评估ALL表型测定的可重复性。样本通过24小时快递服务在各实验室之间运送，并根据间接免疫荧光MoAb反应性分类如下：B系ALL（BA-1+T-MCS-2-）；T系ALL（T+BA-1-MCS-2-）；髓系抗原ALL（MCS-2+BA-1-CALLA-T-）和未分类ALL（BA-1-MCS-2-CALLA-T-）。还进行了表面免疫球蛋白（sIg）、细胞质免疫球蛋白（cIg）、绵羊红细胞花环形成（E）和核末端脱氧核苷酸转移酶（TdT）的传统标志物研究。在有足够标志物数据允许分类的病例中，90%（18/20）被不同实验室进行了相同分类，且这种一致性具有统计学意义（p小于0.05）。除BA-2、cIg和TdT测定外，各实验室之间对于单个MoAb和传统标志物分析的一致性对于所有标志物均具有统计学意义（p小于0.05）。7例不一致的BA-2病例中有6例代表BA-2+评估，在样本运送后随后出现BA-2-结果。这些发现提示BA-2抗原对运输条件不稳定。在涉及其他MoAbs的5/5不一致结果中观察到运输后从阳性到阴性评估的类似模式。各实验室之间对于cIg和TdT测定的不一致意味着细胞质或核抗原的检测可能比细胞表面抗原标志物分析更容易受到主观解释的影响。我们的发现提示，使用针对细胞表面抗原的MoAbs进行免疫荧光标志物研究总体上具有高度可重复性。我们的结果还表明，样本储存条件和靶抗原的细胞定位是可能影响ALL表型测定可重复性的重要变量。
