使用单克隆抗体测定大鼠血浆中的心房肽。
The use of a monoclonal antibody to measure plasma atriopeptins in rat.
作者信息
John A, Stasch J P, Neuser D, Hirth C, Morich F J
出版信息
Life Sci. 1986 Jun 2;38(22):1991-7. doi: 10.1016/0024-3205(86)90146-3.
A monoclonal antibody with specificity for atrial natriuretic peptides (ANP) was produced, that can be used for the radioimmunological determination of ANP-immunoreactivity (ANP-IR) in rat plasma. The antibody recognizes atriopeptin I, II, III, as well as alpha-hANP and alpha-hANP fragment (7-28) and does not crossreact with ANP-fragments (13-28) and (18-28). Plasma levels of ANP-IR in conscious Wistar rats were determined before and after volume-loading. Basal plasma levels of ANP-IR were 108 +/- 12 pg/ml, and after volume-loading increased to 800 +/- 59 pg/ml.
制备了一种对心房利钠肽（ANP）具有特异性的单克隆抗体，该抗体可用于放射免疫法测定大鼠血浆中的ANP免疫反应性（ANP-IR）。该抗体可识别心房肽I、II、III以及α-hANP和α-hANP片段（7-28），且不与ANP片段（13-28）和（18-28）发生交叉反应。测定了清醒Wistar大鼠容量负荷前后血浆中ANP-IR的水平。ANP-IR的基础血浆水平为108±12 pg/ml，容量负荷后升高至800±59 pg/ml。
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