Anastasaki Eirini, Drizou Fryni, Milonas Panagiotis G
Laboratory of Biological Control, Department of Entomology, Benaki Phytopathological Institute, 8 S. Delta street, 14561, Kifissia, Greece.
Division of Plant and Crop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, UK.
J Chem Ecol. 2018 Mar;44(3):288-298. doi: 10.1007/s10886-018-0929-1. Epub 2018 Feb 6.
In response to attack by herbivorous insects, plants produce semiochemicals for intra- and interspecific communication. The perception of these semiochemicals by conspecifics of the herbivore defines their choice for oviposition and feeding. We aimed to investigate the role of herbivore-induced plant volatiles (HIPVs) by Tuta absoluta larvae on the oviposition choice of conspecific females on tomato plants. We performed two- choice and non-choice bioassays with plants damaged by larvae feeding and intact control plants. We also collected headspace volatiles of those plants and tested the response of female antennae on those blends with Gas Chromatography- Electro-Antennographical Detection (GC-EAD). In total 55 compounds were collected from the headspace of T. absoluta larvae-infested plants. Our results show that female moths preferred to oviposit on intact control plants instead of damaged ones. Herbivory induced the emission of hexanal, (Ζ)-3-hexen-1-ol, (E)-β-ocimene, linalool, (Z)-3-hexenyl butanoate, methyl salicylate, indole, nerolidol, guaidiene-6,9, β-pinene, β-myrcene, α-terpinene, hexenyl hexanoate, β-elemene, β-caryophyllene and (Ε-Ε)- 4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), one unidentified sesquiterpene and three unknown compounds. In Electroantennographic (EAG) assays, the antennae of T. absoluta females responded to hexanal, (Ζ)-3-hexen-1-ol, methyl salicylate and indole. The antennae of T. absoluta females exhibited a dose-response in EAG studies with authentic samples. Strong EAG responses were obtained for compounds induced on damaged tomato plants, as well as in nonanal, a compound emitted by both infested and control plants. These compounds could be utilized in integrated pest management of T. absoluta.
作为对植食性昆虫攻击的响应，植物会产生用于种内和种间交流的信息化合物。植食性昆虫的同种个体对这些信息化合物的感知决定了它们的产卵和取食选择。我们旨在研究番茄潜叶蛾幼虫诱导的植物挥发物（HIPVs）对同种雌性在番茄植株上产卵选择的作用。我们用幼虫取食损伤的植株和完整的对照植株进行了二选一和无选择生物测定。我们还收集了这些植株的顶空挥发物，并用气相色谱 - 触角电位检测（GC - EAD）测试了雌性触角对这些混合物的反应。从番茄潜叶蛾幼虫侵染的植株顶空中总共收集到55种化合物。我们的结果表明，雌蛾更喜欢在完整的对照植株上而不是受损植株上产卵。食草作用诱导了己醛、（Z）-3-己烯-1-醇、（E）-β-罗勒烯、芳樟醇、（Z）-3-己烯基丁酸酯、水杨酸甲酯、吲哚倍半萜烯、愈创木二烯-6,9、β-蒎烯、β-月桂烯、α-萜品烯、己烯基己酸酯、β-榄香烯、β-石竹烯和（E,E）-4,8,12-三甲基-1,3,7,11-十三碳四烯（TMTT）、一种未鉴定的倍半萜烯和三种未知化合物的释放。在触角电位（EAG）测定中，番茄潜叶蛾雌蛾的触角对己醛、（Z）-3-己烯-1-醇、水杨酸甲酯和吲哚有反应。在使用纯品的EAG研究中，番茄潜叶蛾雌蛾的触角表现出剂量反应。对于在受损番茄植株上诱导产生的化合物以及在侵染植株和对照植株都释放的壬醛，触角都产生了强烈的EAG反应。这些化合物可用于番茄潜叶蛾的综合虫害管理。
