Arce Carla C M, Machado Ricardo A R, Ribas Natália S, Cristaldo Paulo F, Ataíde Lívia M S, Pallini Ângelo, Carmo Flávia M, Freitas Leandro G, Lima Eraldo
Department of Entomology, Universidade Federal de Viçosa, Viçosa, Brazil.
Institute of Plant Sciences, University of Bern, Bern, Switzerland.
J Chem Ecol. 2017 Feb;43(2):120-128. doi: 10.1007/s10886-016-0810-z. Epub 2017 Jan 13.
The outcome of plant-mediated interactions among herbivores from several feeding guilds has been studied intensively. However, our understanding on the effects of nematode root herbivory on leaf miner oviposition behavior and performance remain limited. In this study, we evaluated whether Meloidogyne incognita root herbivory affects Tuta absoluta oviposition preference on Solanum lycopersicum plants and the development of the resulting offspring. To investigate the M. incognita-herbivory induced plant systemic responses that might explain the observed biological effects, we measured photosynthetic rates, leaf trypsin protease inhibitor activities, and analyzed the profile of volatiles emitted by the leaves of root-infested and non-infested plants. We found that T. absoluta females avoided laying eggs on the leaves of root-infested plants, and that root infestation negatively affected the pupation process of T. absoluta. These effects were accompanied by a strong suppression of leaf volatile emissions, a decrease in photosynthetic rates, and an increase in the activity of leaf trypsin protease inhibitors. Our study reveals that root attack by nematodes can shape leaf physiology, and thereby increases plant resistance.
来自几个取食类群的植食性动物之间通过植物介导的相互作用的结果已得到深入研究。然而，我们对线虫根部取食对番茄潜叶蛾产卵行为和生长性能的影响的了解仍然有限。在本研究中，我们评估了南方根结线虫的根部取食是否会影响番茄潜叶蛾在番茄植株上的产卵偏好以及由此产生的后代的发育。为了研究南方根结线虫取食诱导的植物系统反应，这些反应可能解释观察到的生物学效应，我们测量了光合速率、叶片胰蛋白酶蛋白酶抑制剂活性，并分析了受根侵染和未受根侵染的植物叶片释放的挥发物谱。我们发现，番茄潜叶蛾雌虫避免在受根侵染的植物叶片上产卵，并且根侵染对番茄潜叶蛾的化蛹过程有负面影响。这些影响伴随着叶片挥发物排放的强烈抑制、光合速率的降低以及叶片胰蛋白酶蛋白酶抑制剂活性的增加。我们的研究表明，线虫对根部的攻击可以塑造叶片生理，从而增强植物抗性。
