Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China.
Department of Cardiology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China.
J Cardiovasc Transl Res. 2019 Feb;12(1):37-44. doi: 10.1007/s12265-018-9791-3. Epub 2018 Feb 5.
Exosomes are small-sized vesicles that can be released from cells into the serum. Exosomes play important roles in regulating many biological processes including cell proliferation, apoptosis, cell cycle, and metabolism. However, the roles and mechanisms of plasma exosomes in the apoptosis of rat H9C2 cardiomyocytes are largely unknown. In this study, we isolated plasma exosomes as confirmed by the marker protein CD63. Using flow cytometry and western blot analysis, we found that exosomes attenuated hydrogen peroxide (HO)-induced apoptosis and improved survival of rat H9C2 cardiomyocytes. Furthermore, the anti-apoptosis effects of serum exosomes in rat H9C2 cardiomyocytes were mediated by the activation of ERK1/2 signaling pathway. These data indicated that plasma exosomes had the protective effects against cardiomyocyte apoptosis and might be a novel therapy strategy for myocardial injury.
外泌体是可以从细胞释放到血清中的小囊泡。外泌体在调节许多生物过程中发挥着重要作用，包括细胞增殖、细胞凋亡、细胞周期和代谢。然而，血浆外泌体在大鼠 H9C2 心肌细胞凋亡中的作用和机制在很大程度上尚不清楚。在这项研究中，我们分离了血浆外泌体，这一点通过标记蛋白 CD63 得到了证实。通过流式细胞术和 Western blot 分析，我们发现外泌体可减轻过氧化氢（H2O2）诱导的心肌细胞凋亡并提高大鼠 H9C2 心肌细胞的存活率。此外，血清外泌体在大鼠 H9C2 心肌细胞中的抗凋亡作用是通过 ERK1/2 信号通路的激活来介导的。这些数据表明，血浆外泌体具有保护心肌细胞免受凋亡的作用，可能是心肌损伤的一种新的治疗策略。
