Yu Xiao-Zhang, Fan Wei-Jia, Lin Yu-Juan, Zhang Fei-Fei, Gupta Dharmendra K
The Guangxi Key Laboratory of Theory & Technology for Environmental Pollution Control, College of Environmental Science & Engineering, Guilin University of Technology, 541004, Guilin, People's Republic of China.
Institut für Radioökologie und Strahlenschutz (IRS), Gottfried Wilhelm Leibniz Universität Hannover, Herrenhäuser Str. 2, 30419, Hannover, Germany.
Ecotoxicology. 2018 Apr;27(3):325-335. doi: 10.1007/s10646-018-1897-5. Epub 2018 Feb 5.
Phenylalanine ammonia-lyase (PAL) is one of the principle enzymes involved in plant's secondary metabolism. Expression of individual isogene from the PAL gene family is variable with species of plants in responses to different stresses. In this study, transcriptome analysis of the PAL gene family in rice seedlings exposed to potassium chromate Cr(VI) or chromium nitrate Cr(III) was conducted using Agilent 44K rice microarray and real-time quantitative RT-PCR. Uptake and accumulation of both Cr species by rice seedlings and their effect on PAL activity were also determined. Three days of Cr exposure led to significant accumulation of Cr in plant tissues, but majority being in roots rather than shoots. Changes of PAL activities in rice tissues were evident from both Cr treatments. Individual isogene from the rice PAL gene family was expressed differentially in response to both Cr variants. Comparing gene expression between two Cr treatments, only osPAL2 and osPAL4 genes were expressed in similar patterns. Also, gene expression pattern was inconsistent in both plant tissues. Results indicated that expression of individual isoform from the rice PAL gene family is tissue, and stimulus specific under different Cr exposure, suggesting their different detoxification strategies for decreasing or eliminating Cr stresses.
苯丙氨酸解氨酶（PAL）是参与植物次生代谢的主要酶之一。PAL基因家族中单个同基因的表达会因植物种类不同而在应对不同胁迫时有所变化。在本研究中，利用安捷伦44K水稻微阵列和实时定量逆转录聚合酶链式反应（RT-PCR）对暴露于铬酸钾Cr(VI)或硝酸铬Cr(III)的水稻幼苗中PAL基因家族进行了转录组分析。同时还测定了水稻幼苗对两种铬形态的吸收和积累及其对PAL活性的影响。铬暴露三天导致植物组织中铬大量积累，但大部分集中在根部而非地上部分。两种铬处理均使水稻组织中的PAL活性发生明显变化。水稻PAL基因家族中的单个同基因在应对两种铬变体时表达存在差异。比较两种铬处理之间的基因表达，只有osPAL2和osPAL4基因以相似模式表达。此外，两种植物组织中的基因表达模式也不一致。结果表明，水稻PAL基因家族中单个同工型的表达具有组织特异性，且在不同铬暴露条件下对刺激的反应不同，这表明它们在减轻或消除铬胁迫方面有不同的解毒策略。
