Grupo de Ciencias Básicas Médicas, School of Medicine, Universidad de Los Andes, Bogotá, Colombia.
Scientific Research Unit, Hospital Militar Central, Bogotá, Colombia.
Am J Trop Med Hyg. 2018 Mar;98(3):717-723. doi: 10.4269/ajtmh.17-0417. Epub 2018 Feb 1.
Achalasia is a motility disorder of the esophagus that might be secondary to a chronic infection. Several studies have investigated esophageal achalasia in patients with Chagas disease (CD) in Latin America, but no related studies have been performed in Colombia. The goals of the present study were to determine the presence of anti- antibodies in patients with esophageal achalasia who visited a referral hospital in Bogotá, Colombia, and to detect the presence of the parasite and its discrete typing units (DTUs). This cross-sectional study was conducted in adult patients (18-65 years old) who were previously diagnosed with esophageal achalasia and from whom blood was drawn to assess antibodies against using four different serological tests. DNA was detected by conventional polymerase chain reaction (cPCR) and quantitative polymerase chain reaction (qPCR). In total, 38 patients, with an average age of 46.6 years (standard deviation of ±16.2) and comprising 16 men and 22 women, were enrolled. Five (13.15%) patients were found to be positive for anti- antibodies by indirect immunofluorescence assay (IFA), and two patients who were negative according to IFA were reactive by both enzyme-linked immunosorbent assay and immunoblot (5.3%). Parasite DNA was detected in two of these seven patients by cPCR and in one of these by qPCR. The parasite DTU obtained was TcI. In summary, this study identified in Colombian patients with esophageal achalasia, indicating that digestive compromise could also be present in patients with chronic CD.
贲门失弛缓症是一种食管动力障碍性疾病，可能继发于慢性感染。几项研究已经在拉丁美洲的恰加斯病（CD）患者中调查了食管贲门失弛缓症，但在哥伦比亚尚未进行相关研究。本研究的目的是确定在波哥大的一家转诊医院就诊的食管贲门失弛缓症患者中是否存在抗抗体，并检测寄生虫及其离散型单位（DTUs）的存在。这项横断面研究纳入了 38 名先前被诊断为食管贲门失弛缓症的成年患者（18-65 岁），采集血液以使用四种不同的血清学检测方法评估针对的抗体。采用常规聚合酶链反应（cPCR）和实时聚合酶链反应（qPCR）检测 DNA。共有 38 名患者入组，平均年龄为 46.6 岁（标准差为±16.2），包括 16 名男性和 22 名女性。5 名（13.15%）患者间接免疫荧光法（IFA）检测抗抗体阳性，2 名 IFA 阴性患者酶联免疫吸附试验和免疫印迹法均呈阳性（5.3%）。cPCR 在这 7 名患者中的 2 名中检测到寄生虫 DNA，qPCR 在其中 1 名中检测到。获得的寄生虫 DTU 为 TcI。总之，本研究在哥伦比亚的食管贲门失弛缓症患者中发现了，这表明慢性 CD 患者也可能存在消化功能障碍。
