Daniel W C, Nair S V, Bluestein J
Acta Cytol. 1979 May-Jun;23(3):222-6.
Freshly expectorated sputum from a 52-year-old man with extensive pulmonary blastomycosis was examined by light and electron microscopy. Wet preparations of the sputum, stained with 1% aqueous cresyl violet, revealed numerous thick-walled, spherical blastospores and occasional budding forms. Ultrastructurally, the blastospores revealed a double-layered cell wall, the peripheral portion of which consisted of a thick, densely staining lamellar material of variable thickness, suggestive of the glycocalyx of bacterial cells. Mitochrondria and other organelles were easily recognized, but distinct nuclei were not observed. Retraction of the cell membrane and cytoplasmic mass from the cell wall was a common finding. Results of this case study suggest that electron microscopy of sputum may serve as an important adjunct to conventional staining techniques for the presumptive diagnosis of blastomycosis. Similar studies of other fungi in respiratory tract secretions may be helpful in providing more precise morphologic identification.
对一名患有广泛性肺芽生菌病的52岁男性的新鲜咳出痰进行了光镜和电镜检查。用1%的甲酚紫水溶液染色的痰湿片显示出大量厚壁球形芽生孢子以及偶尔的出芽形式。超微结构上，芽生孢子显示出双层细胞壁，其外周部分由厚度可变的厚且染色致密的层状物质组成，提示为细菌细胞的糖萼。线粒体和其他细胞器易于识别，但未观察到明显的细胞核。细胞膜和细胞质团从细胞壁退缩是常见现象。该病例研究结果表明，痰的电镜检查可作为传统染色技术的重要辅助手段，用于芽生菌病的初步诊断。对呼吸道分泌物中其他真菌进行类似研究可能有助于提供更精确的形态学鉴定。
