Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
Anal Chim Acta. 2018 May 12;1007:33-39. doi: 10.1016/j.aca.2017.12.005. Epub 2017 Dec 27.
This paper describes a novel rotational paper-based analytical device (RPAD) to implement multi-step electrochemiluminescence (ECL) immunoassays. The integrated paper-based rotational valves can be easily controlled by rotating paper discs manually and this advantage makes it user-friendly to untrained users to carry out the multi-step assays. In addition, the rotational valves are reusable and the response time can be shortened to several seconds, which promotes the rotational paper-based device to have great advantages in multi-step operations. Under the control of rotational valves, multi-step ECL immunoassays were conducted on the rotational device for the multiplexed detection of carcinoembryonic antigen (CEA) and prostate specific antigen (PSA). The rotational device exhibited excellent analytical performance for CEA and PSA, and they could be detected in the linear ranges of 0.1-100 ng mL and 0.1-50 ng mL with detection limits down to 0.07 ng mL and 0.03 ng mL, respectively, which were within the ranges of clinical concentrations. We hope this technique will open a new avenue for the fabrication of paper-based valves and provide potential application in clinical diagnostics.
本文描述了一种新颖的基于纸的旋转分析装置 (RPAD)，用于实现多步电化学发光 (ECL) 免疫分析。集成的基于纸的旋转阀可以通过手动旋转纸盘轻松控制，这一优势使得未经训练的用户也可以方便地进行多步检测。此外，旋转阀是可重复使用的，并且响应时间可以缩短到几秒钟，这使得基于纸的旋转装置在多步操作中具有很大的优势。在旋转阀的控制下，在旋转装置上进行了多步 ECL 免疫分析，用于同时检测癌胚抗原 (CEA) 和前列腺特异性抗原 (PSA)。该旋转装置对 CEA 和 PSA 表现出优异的分析性能，其线性检测范围分别为 0.1-100ng/mL 和 0.1-50ng/mL，检测限分别低至 0.07ng/mL 和 0.03ng/mL，均处于临床浓度范围内。我们希望这项技术将为基于纸的阀门的制造开辟新的途径，并为临床诊断提供潜在的应用。
