State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Institute of Nanochemistry and Nanobiology, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Anal Chem. 2022 Feb 15;94(6):3005-3012. doi: 10.1021/acs.analchem.1c05383. Epub 2022 Feb 1.
Owing to the characteristics of high throughput, high flexibility, and convenient separation of the sensing and reporting reactions, the bipolar electrode (BPE) shows great potential in clinical analysis. However, there are some difficulties in the combination of BPEs and multiplex electrochemiluminescence (ECL) biosensing, such as the need for small sample consumption, multistep operations, and separated sample loading. In this paper, a microfluidic BPE array chip was fabricated toward multiplex detection of cancer biomarkers. With a special channel structure and the difference in flow resistance of channels of different sizes, the direction of liquid flow was successfully controlled. In this way, rapid and automatic multiplex sampling was achieved on the array, which would help improve the sensing efficiency and reduce the reagent consumption. The ECL BPE array chip served as an immunosensor for multiple prostate cancer biomarkers including prostate-specific antigen (PSA), interleukin-6 (IL-6), and prostate-specific membrane antigen (PSMA). The microfluidic BPE chip shows good reproducibility and high sensitivity. The limits of detection for PSA, IL-6, and PSMA are 0.093 ng/mL, 0.061 pg/mL, and 0.059 ng/mL, respectively. It also exhibits excellent performance in real sample analysis. The integrated ECL BPE array shows a good application prospect in clinical sensing of cancer biomarkers, as well as point-of-care testing.
由于双极电极 (BPE) 具有高通量、高灵活性和传感与报告反应易于分离的特点，因此在临床分析中具有很大的潜力。然而，BPE 与多路电化学发光 (ECL) 生物传感的结合存在一些困难，例如需要小样本消耗、多步操作和分离的样本加载。在本文中，我们制备了一种微流控 BPE 阵列芯片，用于多路检测癌症生物标志物。通过特殊的通道结构和不同尺寸通道的流动阻力差异，成功控制了液体的流向。这样，就可以在阵列上实现快速和自动的多路采样，从而有助于提高传感效率并减少试剂消耗。ECL BPE 阵列芯片作为一种用于多种前列腺癌生物标志物（包括前列腺特异性抗原 (PSA)、白细胞介素-6 (IL-6) 和前列腺特异性膜抗原 (PSMA)）的免疫传感器。微流控 BPE 芯片具有良好的重现性和高灵敏度。PSA、IL-6 和 PSMA 的检测限分别为 0.093 ng/mL、0.061 pg/mL 和 0.059 ng/mL。它在实际样品分析中也表现出优异的性能。集成的 ECL BPE 阵列在癌症生物标志物的临床传感以及即时检测中具有很好的应用前景。
