CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
J Environ Sci (China). 2018 Jan;63:116-125. doi: 10.1016/j.jes.2017.09.010. Epub 2017 Sep 29.
A simple method for determining 33 pesticides with a wide polarity range (logK 0.6-4.5) in aquatic products was developed based on LC-MS/MS. The target analytes included three types of widely used pesticides: insecticides, fungicides and herbicides. Based on the optimization of ultrasonic assisted extraction and GPC clean-up procedures, the matrix effect, extraction recoveries and LOD were improved distinctively. LOQ of this method was below 0.5ng/g for all pesticides, which is superior to values in the literature, and the matrix effect was reduced effectively (-14.7% to 7.5%). The method was successfully applied to investigate the pesticide residue levels of twenty-five samples including seven common kinds of fishes from Northeast China. The results showed that all targeted pesticides were present in the fish samples; however, their levels were low, except for atrazine, linuron, ethoprophos, tetrachlorvinphos, acetochlor and fenthion. Atrazine and linuron caught our attention because the concentrations of atrazine in fish samples from Liaoning province were in the range of 0.5-8ng/g (w/w) with mean concentration of 2.3ng/g, which were far above those of other pesticides. The levels of linuron were in the range of 0.6-6ng/g (mean concentration 2.8ng/g), which were the highest among all targeted pesticides in the Inner Mongolia. This is the first systematic investigation on the characteristics and levels of these pesticides in aquatic products from northeast China. Considering their toxicity and bioaccumulation, the potential risk of atrazine and linuron from consuming aquatic products should be paid more attention.
建立了一种基于 LC-MS/MS 的简单方法，用于测定水产品中具有较宽极性范围（logK 0.6-4.5）的 33 种农药。目标分析物包括三种广泛使用的农药：杀虫剂、杀菌剂和除草剂。通过对超声辅助提取和 GPC 净化程序的优化，显著改善了基质效应、提取回收率和 LOD。该方法对所有农药的 LOQ 均低于 0.5ng/g，优于文献值，并且有效降低了基质效应（-14.7%至 7.5%）。该方法成功应用于调查来自中国东北地区的 25 个样品（包括 7 种常见鱼类）中的农药残留水平。结果表明，所有目标农药均存在于鱼样中，但浓度均较低，除莠剂、利谷隆、乙草胺、氯硫磷、乙酮和倍硫磷外。莠剂和利谷隆引起了我们的注意，因为辽宁省鱼类样本中莠剂的浓度范围为 0.5-8ng/g（w/w），平均浓度为 2.3ng/g，远高于其他农药。利谷隆的浓度范围为 0.6-6ng/g（平均浓度 2.8ng/g），在内蒙古所有目标农药中最高。这是首次对来自中国东北的水产品中这些农药的特征和水平进行系统调查。考虑到它们的毒性和生物累积性，应该更加关注食用水产品中莠剂和利谷隆的潜在风险。
