Athanasiou Labrini V, Katsoulos Panagiotis D, Katsogiannou Eleni G, Polizopoulou Zoe S, Diamantaki Myrto, Kamatsos Constantinos, Christodoulopoulos Georgios
Department of Medicine, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece.
Clinic of Farm Animals, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Vet Clin Pathol. 2018 Jun;47(2):284-288. doi: 10.1111/vcp.12581. Epub 2018 Feb 6.
Urine pH is an integral part of a complete urinalysis, and is commonly measured in veterinary practice using semiquantitative reagent strips.
The aim of this study was to compare the urine pH of dogs and sheep, using visual interpretation of dipstick reactions, and using a pH-meter as the reference method. Agreement between the 2 methods was also assessed. An additional objective was to compare the urine pH before and after centrifugation.
A total of 50 voided urine samples from sheep and 52 from dogs were collected into sterile containers. For pH measurements, 2 methods were used, a pH-meter and urine dipstick reagent pads. Measurements were performed using urine samples before (whole urine) and after centrifugation (urine supernatant). For comparison of the 2 methods, Passing and Bablok regression analysis and Bland-Altman plots were used.
The equation created to assess agreement between the 2 methods in dogs showed a constant bias at -0.14 and a positive proportional bias at 0.98. From a clinical standpoint, total bias was below and above the maximum acceptable bias in sheep and dogs, respectively. Clinically acceptable bias was also found using centrifuged urine samples in sheep, but the urine pH values before and after centrifugation were nearly identical in dogs.
Urine dipstick reagent pads and pH-meters can be used interchangeably to determine urine pH in sheep without needing centrifugation. In contrast, pH-meters provide more accurate pH measurements than urine dipstick pads in canine urine, which is not improved by centrifugation.
尿液pH值是完整尿液分析的一个组成部分，在兽医实践中通常使用半定量试剂条进行测量。
本研究的目的是使用试纸条反应的视觉判读，并以pH计作为参考方法，比较犬和绵羊的尿液pH值。还评估了两种方法之间的一致性。另一个目的是比较离心前后的尿液pH值。
共收集了50份绵羊的新鲜尿液样本和52份犬的新鲜尿液样本，放入无菌容器中。对于pH测量，使用了两种方法，即pH计和尿液试纸条试剂垫。使用尿液样本在离心前（全尿）和离心后（尿液上清液）进行测量。为了比较两种方法，使用了Passing和Bablok回归分析以及Bland-Altman图。
用于评估犬的两种方法之间一致性的方程显示，恒定偏差为-0.14，正比例偏差为0.98。从临床角度来看，总偏差分别低于和高于绵羊和犬的最大可接受偏差。在绵羊中使用离心后的尿液样本也发现了临床可接受的偏差，但犬离心前后的尿液pH值几乎相同。
尿液试纸条试剂垫和pH计可互换使用以确定绵羊的尿液pH值，无需离心。相比之下，pH计在犬尿液中提供的pH测量比尿液试纸条更准确，离心并不能改善这一点。
