通过聚丙烯酰胺凝胶等电聚焦对马来西亚血吸虫、湄公血吸虫和日本血吸虫进行同工酶分析。

Isoenzyme analyses of Malaysian Schistosoma, S. mekongi and S. japonicum by isoelectric focusing in polyacrylamide gel.

作者信息

Viyanant V, Upatham E S

出版信息

Southeast Asian J Trop Med Public Health. 1985 Dec;16(4):539-45.

Abstract

Isoenzyme patterns of adult Malaysian Schistosoma, S. mekongi and S. japonicum strains were analysed by isoelectric focusing (IEF) in polyacrylamide gel. Enzyme patterns obtained from Malaysian Schistosoma homogenates differed from those of S. mekongi and S. japonicum strains. Malaysian Schistosoma was found to differ from S. japonicum by 8 enzymes, namely phosphoglucomutase, phosphoglucoisomerase, malate dehydrogenase, acid phosphatase, hydroxy-butyrate dehydrogenase, hexokinase and alkaline phosphatase, and from S. mekongi by phosphoglucomutase, malate dehydrogenase, aldolase and alkaline phosphatase. These results and the distinct biology of the parasite suggest that Malaysian Schistosoma is a new species in the S. japonicum complex.

摘要

采用聚丙烯酰胺凝胶等电聚焦（IEF）技术分析了马来西亚成人血吸虫、湄公血吸虫和日本血吸虫菌株的同工酶模式。从马来西亚血吸虫匀浆中获得的酶模式与湄公血吸虫和日本血吸虫菌株的酶模式不同。发现马来西亚血吸虫与日本血吸虫在8种酶上存在差异，即磷酸葡萄糖变位酶、磷酸葡萄糖异构酶、苹果酸脱氢酶、酸性磷酸酶、羟基丁酸脱氢酶、己糖激酶和碱性磷酸酶，与湄公血吸虫在磷酸葡萄糖变位酶、苹果酸脱氢酶、醛缩酶和碱性磷酸酶上存在差异。这些结果以及该寄生虫独特的生物学特性表明，马来西亚血吸虫是日本血吸虫复合种中的一个新物种。