Rollinson D, Southgate V R
Trans R Soc Trop Med Hyg. 1979;73(6):667-72. doi: 10.1016/0035-9203(79)90017-8.
38 population samples of snails of the Bulinus africanus group, collected from three separate areas of Tanzania, have been examined. Enzymes in crude digestive gland extracts of individual snails have been analysed by isoelectric focusing in polyacrylamide gels. The enzymes studied were: malate dehydrogenase (MDH); phosphoglucomutase (PGM); glucosephosphate isomerase (GPI); acid phosphatase (AcP) and hydroxybutyrate dehydrogenase (HBDH). Samples of B. nasutus were clearly differentiated from other species and enzyme differences were apparent between samples from the lake and coastal areas. Similarly, although clear distinctions could not always be made, samples of B. africanus, B. globosus and B. ugandae were characterized by their enzyme types. Individual variation was detected within populations and the significance of enzyme polymorphisms in relation to identification has been considered. No correlation was found between snail enzyme type and susceptibility to Schistosoma haematobium or S. bovis.
对从坦桑尼亚三个不同地区采集的38份非洲小泡螺种群样本进行了检查。通过在聚丙烯酰胺凝胶中进行等电聚焦,分析了单个蜗牛粗消化腺提取物中的酶。所研究的酶包括:苹果酸脱氢酶(MDH);磷酸葡萄糖变位酶(PGM);葡萄糖磷酸异构酶(GPI);酸性磷酸酶(AcP)和羟基丁酸脱氢酶(HBDH)。鼻形小泡螺样本与其他物种明显不同,湖泊地区和沿海地区的样本之间酶存在差异。同样,尽管并非总能明确区分,但非洲小泡螺、球小泡螺和乌干达小泡螺样本具有各自的酶类型特征。在种群内部检测到了个体变异,并考虑了酶多态性在识别方面的意义。未发现蜗牛酶类型与对埃及血吸虫或牛血吸虫易感性之间存在相关性。