US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid-Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804, USA.
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid-Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804, USA.
Regul Toxicol Pharmacol. 2018 Apr;94:124-143. doi: 10.1016/j.yrtph.2018.01.019. Epub 2018 Feb 6.
Ecological risk assessments are often limited in their ability to consider metabolic transformations for fish species due to a lack of data. When these types of evaluations are attempted they are often based on parent chemical only, or by assuming similarity to available mammalian metabolic pathways. The metabolism maps for five pesticides (fluazinam, halauxifen-methyl, kresoxim-methyl, mandestrobin, and tolclofos-methyl) were compared across three species. A rapid and transparent process, utilizing a database of systematically collected information for rat, goat, and fish (bluegill or rainbow trout), and using data evaluation tools in the previously described metabolism pathway software system MetaPath, is presented. The approach demonstrates how comparisons of metabolic maps across species are aided by considering the sample matrix in which metabolites were quantified for each species, differences in analytical methods used to identify metabolites in each study, and the relative amounts of metabolites quantified. By incorporating these considerations, more extensive rat and goat metabolism maps were found to be useful predictors of the more limited metabolism of the five pesticides in fish.
生态风险评估通常由于缺乏数据而无法考虑鱼类的代谢转化，从而限制了其能力。在尝试进行这些类型的评估时，它们通常仅基于母体化学物质，或者假设与可用的哺乳动物代谢途径相似。本文比较了 5 种农药（氟唑菌酰胺、halauxifen-methyl、肟菌酯、螺甲螨酯和唑草酮）在 3 个物种中的代谢图谱。利用数据库（包含大鼠、山羊和鱼类（蓝鳃太阳鱼或虹鳟）的系统收集信息）和先前描述的代谢途径软件系统 MetaPath 中的数据评估工具，提出了一种快速透明的方法。该方法演示了如何通过考虑代谢物定量的样本基质、每个研究中用于鉴定代谢物的分析方法的差异以及定量代谢物的相对量，来帮助比较跨物种的代谢图谱。通过纳入这些考虑因素，发现更广泛的大鼠和山羊代谢图谱可更有效地预测鱼类中 5 种农药更有限的代谢。
