School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.
Key Lab of Mariculture and Enhancement of Zhejiang Province, Zhejiang Marine Fisheries Research Institute, Zhoushan 316021, China.
Molecules. 2023 Jul 15;28(14):5430. doi: 10.3390/molecules28145430.
Valnemulin (VML) is a semi-synthetic pleuromutilin derivative widely used to treat animal bacterial diseases. However, no study has comprehensively evaluated VML metabolism in aquatic animals, including crucian carp. This study aimed to investigate VML metabolism in crucian carp. VML metabolites in crucian carp were quantified via intraperitoneal injection and analyzed via ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Three VML metabolites were detected in crucian carp via ultra-performance liquid chromatography-tandem quadrupole and time-of-flight mass spectrometry (UPLC-Q-TOF/MS) structural analysis. The enrichment and metabolism rules of the metabolites were summarized based on tissue distribution and concentration changes of the three metabolites. The metabolites were mainly found in the liver at 0.1 h after VML injection. The levels of the metabolites were abundant in the bile from 4 h to 12 h and in the skin after 72 h. The levels of the metabolites in the bile first increased, then decreased. The metabolism in the liver was completed at 72 h. The metabolites were detected in the skin following a 72 h period, which increased with time.
沃尼妙林（VML）是一种广泛用于治疗动物细菌性疾病的半合成截短侧耳素衍生物。然而，目前还没有研究全面评估水生动物（包括鲫鱼）中的 VML 代谢情况。本研究旨在研究 VML 在鲫鱼中的代谢情况。通过腹腔注射，采用超高效液相色谱-串联质谱法（UPLC-MS/MS）对鲫鱼中的 VML 代谢物进行定量分析。通过超高效液相色谱-四极杆飞行时间串联质谱法（UPLC-Q-TOF/MS）结构分析，在鲫鱼中检测到 3 种 VML 代谢物。基于 3 种代谢物的组织分布和浓度变化，总结了代谢物的富集和代谢规律。VML 注射后 0.1 h，代谢物主要在肝脏中发现。4 至 12 h 胆汁中代谢物水平丰富，72 h 后在皮肤中。胆汁中代谢物的水平先增加后减少，72 h 后在肝脏中完成代谢。72 h 后在皮肤中检测到代谢物，其含量随时间增加。
