Water Research and Technologies Center (CERTE), Laboratory of Wastewater Treatment, University of Carthage, P.O. Box 273, 8020 Soliman, Tunis, Tunisia.
Laboratoire des Maladies Transmissibles et des Substances Biologiquement Actives, Faculté de Pharmacie, Université de Monastir, Tunisia.
Microb Pathog. 2018 Mar;116:328-334. doi: 10.1016/j.micpath.2018.01.042. Epub 2018 Jan 31.
The virulence properties of Candida spp. presents in landfill leachate still unknown until today and they constitutes a serious source of potential danger for humans. We investigate the antifungal susceptibility, production of hydrolytic enzymes and biofilm formation on polystyrene as well as glass in Candida spp. strains isolated from a landfill leachate treatment station in Borj Chakir (Tunisia). 37 yeast strains were isolated belonging to the following species: C. robusta, C. lusitaniae, C. tropicalis, C. krusei. Most isolated yeast strains were resistant to Amphotericin B, produced several hydrolytic enzymes (67.56% produced phospholipase, 86.04% protease, 64.86% esterase) and most of them are able to degrade hemoglobin. All assayed Candida strains have been able to form biofilm on polystyrene depending on the species and strain of Candida. Landfills receiving clinical waste are a potential source of Candida ssp. strains with several virulence properties which allow them to survive in different aquatic biotopes.
目前，垃圾渗滤液中存在的念珠菌的毒力特性仍不清楚，它们对人类构成了严重的潜在危险。我们研究了从突尼斯 Borj Chakir 垃圾渗滤液处理站分离的念珠菌菌株在聚苯乙烯和玻璃上的抗真菌敏感性、水解酶的产生和生物膜的形成。从该处理站共分离出 37 株酵母，分别属于：C. robusta、C. lusitaniae、C. tropicalis 和 C. krusei。大多数分离出的酵母菌株对两性霉素 B 具有耐药性，能产生多种水解酶（67.56%产生磷脂酶，86.04%产生蛋白酶，64.86%产生酯酶），其中大多数能降解血红蛋白。所有测试的念珠菌菌株都能够在聚苯乙烯上形成生物膜，这取决于念珠菌的种类和菌株。接收临床废物的垃圾填埋场是具有多种毒力特性的念珠菌菌株的潜在来源，这些特性使它们能够在不同的水生生物栖息地中存活。
