Department of Food Hygiene and Technology, Veterinary Faculty, University of León, E-24071 León, Spain; Institute of Food Science and Technology, University of León, E-24071 León, Spain.
Department of Food Hygiene and Technology, Veterinary Faculty, University of León, E-24071 León, Spain; Institute of Food Science and Technology, University of León, E-24071 León, Spain.
Food Microbiol. 2018 Jun;72:220-224. doi: 10.1016/j.fm.2017.11.018. Epub 2017 Dec 7.
Methicillin-resistant staphylococci (MRS) are a major concern to public and animal health. Thirty MRS (Staphylococcus aureus, S. cohnii, S. epidermidis, S. haemolyticus, S. hominis, S. lentus, S. lugdunensis, S. sciuri, and S. xylosus) isolates from meat and poultry preparations were tested for antimicrobial susceptibility to 11 antimicrobials (belonging to seven different categories) of clinical significance using both the standard agar disc diffusion method and a commercially available miniaturized system (Sensi Test Gram-positive). It is worth stressing that 16 isolates (53.33%) exhibited an extensively drug-resistant phenotype (XDR). The average number of resistances per strain was 4.67. These results suggest that retail meat and poultry preparations are a likely vehicle for the transmission of multi-drug resistant MRS. Resistance to erythromycin was the commonest finding (76.67% of strains), followed by tobramycin, ceftazidime (66.67%), ciprofloxacin (56.67%) and fosfomycin (53.33%). An agreement (kappa coefficient) of 0.64 was found between the two testing methods. Using the agar disc diffusion as the reference method, the sensitivity, specificity and accuracy of the miniaturized test were 98.44%, 69.44% and 83.33%, respectively. Most discrepancies between the two methods were due to isolates that were susceptible according to the disc diffusion method but resistant according to the miniaturized test (false positives).
耐甲氧西林葡萄球菌（MRS）是公共卫生和动物健康的主要关注点。从肉类和家禽制品中分离出 30 株 MRS（金黄色葡萄球菌、凝固酶阴性葡萄球菌、表皮葡萄球菌、溶血葡萄球菌、人葡萄球菌、缓症葡萄球菌、路邓葡萄球菌、松鼠葡萄球菌和木糖葡萄球菌），使用标准琼脂扩散法和市售的微型化系统（Sensi Test Gram-positive）对 11 种具有临床意义的抗菌药物（属于 7 个不同类别）进行药敏试验。值得强调的是，有 16 株（53.33%）表现出广泛耐药表型（XDR）。每株菌的耐药平均数量为 4.67。这些结果表明，零售肉类和家禽制品可能是传播多药耐药 MRS 的媒介。红霉素耐药是最常见的发现（76.67%的菌株），其次是妥布霉素、头孢他啶（66.67%）、环丙沙星（56.67%）和磷霉素（53.33%）。两种检测方法之间存在 0.64 的一致性（kappa 系数）。以琼脂扩散法为参考方法，微型化试验的灵敏度、特异性和准确性分别为 98.44%、69.44%和 83.33%。两种方法之间的大多数差异是由于根据扩散法敏感但根据微型化试验耐药的分离株（假阳性）造成的。
