Yao Zhiqiu, Si Wenyu, Tian Weiguo, Ye Jing, Zhu Rongfei, Li Xiumei, Ji Shichun, Zheng Qianqian, Liu Ya, Fang Fugui
Anhui Provincial Key Laboratory of Genetic Resources Protection and Biological Breeding in Local Livestock and Poultry, 130 Changjiang West Road, Hefei, Anhui 230036, China; Department of Animal Veterinary Science, College of Animal Sciences and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Department of Animal Veterinary Science, College of Animal Sciences and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Theriogenology. 2018 Apr 15;111:1-8. doi: 10.1016/j.theriogenology.2018.01.013. Epub 2018 Jan 31.
To investigate the effect of gonadotropin-releasing hormone 2-multiple antigen peptide (GnRH2-MAP) on reproductive function. In our study, 20-day-old male rats (n = 90) were randomly allocated to one of three treatment groups: GnRH2-MAP immunization, GnRH2 immunization, and non-immunized control groups. The immunized animals were administered three doses of GnRH2-MAP or GnRH2 vaccines from 0 to 6 weeks at 2-week intervals. The control group only received oil adjuvant. Blood and right testis samples were collected, and the left testis was weighed and its volume was measured at 0, 2, 4, 6, 8, 10 and 12 weeks after the first immunization. The serum antibody titer and testosterone concentration were determined by ELISA, and the right testis samples were collected for histological analysis. The results revealed that the serum of vaccinated rats elicited a significantly higher antibody titer and a lower T concentration compared with the control group two weeks after the first immunization (P < 0.05), but the highest antibody titer and lowest T concentration were found in animals treated with GnRH2-MAP (P < 0.05). The second immunization resulted in a significant decrease in testicular weight and volume (P < 0.05) in both immunized groups compared to the control, but these values were significantly lower in the GnRH2-MAP group than in the GnRH2 group. Furthermore, seminiferous tubules revealed more significant atrophy in the GnRH2-MAP group than in the GnRH2 group, and no sperm were observed in rats of the GnRH2-MAP group. Thus, GnRH2-MAP may be an effective antigen and a potential immunocastration vaccine with higher effectiveness.
为研究促性腺激素释放激素2-多抗原肽（GnRH2-MAP）对生殖功能的影响。在我们的研究中，将20日龄雄性大鼠（n = 90）随机分为三个治疗组之一：GnRH2-MAP免疫组、GnRH2免疫组和未免疫对照组。对免疫动物在0至6周期间每隔2周给予三剂GnRH2-MAP或GnRH2疫苗。对照组仅接受油佐剂。在首次免疫后的0、2、4、6、8、10和12周采集血液和右侧睾丸样本，称量左侧睾丸重量并测量其体积。通过酶联免疫吸附测定法（ELISA）测定血清抗体滴度和睾酮浓度，并采集右侧睾丸样本进行组织学分析。结果显示，与对照组相比，首次免疫两周后接种疫苗大鼠的血清产生了显著更高的抗体滴度和更低的T浓度（P < 0.05），但在用GnRH2-MAP治疗的动物中发现抗体滴度最高且T浓度最低（P < 0.05）。与对照组相比，第二次免疫导致两个免疫组的睾丸重量和体积显著下降（P < 0.05），但GnRH2-MAP组的这些值显著低于GnRH2组。此外，GnRH2-MAP组的生精小管萎缩比GnRH2组更显著，并且在GnRH2-MAP组的大鼠中未观察到精子。因此，GnRH2-MAP可能是一种有效的抗原和具有更高有效性的潜在免疫去势疫苗。
