多途径协同作用改变了山羊对免疫去势疫苗的甾体生成和精子发生。

Multiple-Pathway Synergy Alters Steroidogenesis and Spermatogenesis in Response to an Immunocastration Vaccine in Goat.

机构信息

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.

Key Laboratory of Smart Farming for Agricultural Animals, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

Cells. 2023 Dec 20;13(1):6. doi: 10.3390/cells13010006.

DOI:10.3390/cells13010006

PMID:38201210

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10778245/

Abstract

BACKGROUND

Animal reproduction performance is crucial in husbandry. Immunocastrated animals serve as an ideal animal model for studying testicular function. During androgen suppression, the testis undergoes dramatic developmental and structural changes, including the inhibition of hormone secretion and spermatogenesis.

METHODS

To characterize this process, we investigated the effects of castration using a recombinant and DNA vaccine, and then identified functional genes in the testes of Yiling goats using RNA-seq and WGS. The experimental animals were divided into three groups: the PVAX-asd group (control), PBK-asd-immunized group, and surgically castrated group.

RESULTS

The results demonstrated that the administration of the recombinant PBK-asd vaccine in goats elicited a significant antibody response, and reduced serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH), resulting in smaller scrotal circumferences and decreased sexual desire compared to the control group. In addition, RNA transcriptome sequencing (RNA-seq) analysis of the testes revealed that the biological processes after immunocastration mainly focused on the regulation of cell matrix adhesion, histone acetylation, negative regulation of developmental processes, apoptosis, and activation of the complement system and the thrombin cascade reaction system. Then, we integrated the whole-genome sequencing and testis transcriptome, and identified several candidate genes (, , , , , , , , , ) that influence steroidogenesis secretion and spermatogenesis.

CONCLUSIONS

Multiple pathways and polygenic co-expression participate in the response to castration vaccines, altering hormone secretion and spermatogenesis. Taken together, our atlas of the immunocastration goat testis provides multiple insights into the developmental changes and key factors accompanying androgen suppression, and thus may contribute to understanding the genetic mechanism of testis function. Joint analysis of whole genome sequencing and RNA-seq enables reliable screening of candidate genes, benefiting future genome-assisted breeding of goats.

摘要

背景

动物繁殖性能在畜牧业中至关重要。免疫去势动物是研究睾丸功能的理想动物模型。在雄激素抑制期间，睾丸经历了显著的发育和结构变化，包括激素分泌和精子发生的抑制。

方法

为了描述这一过程，我们使用重组和 DNA 疫苗研究了去势的影响，然后使用 RNA-seq 和 WGS 鉴定了夷陵山羊睾丸中的功能基因。实验动物分为三组：PVAX-asd 组（对照）、PBK-asd 免疫组和手术去势组。

结果

结果表明，重组 PBK-asd 疫苗在山羊中的给药引起了显著的抗体反应，并降低了血清促卵泡激素（FSH）和促黄体生成素（LH），导致阴囊周长减小，性欲降低与对照组相比。此外，睾丸的 RNA 转录组测序（RNA-seq）分析表明，免疫去势后的生物过程主要集中在细胞基质粘附、组蛋白乙酰化、发育过程的负调节、细胞凋亡、补体系统和凝血酶级联反应系统的激活的调节上。然后，我们整合了全基因组测序和睾丸转录组，鉴定了几个影响类固醇分泌和精子发生的候选基因（、、、、、、、、、）。