Guo Junnan, Xu Jinzhong, Zhang Junshi, An Lei
Department of Neurology, Huaihe Hospital of Henan University, Kaifeng, 475000, Henan, China.
Pediatric Surgery, Children's Hospital of Kaifeng City, Kaifeng, 475000, Henan, China.
Toxicol Lett. 2018 May 1;287:1-9. doi: 10.1016/j.toxlet.2018.01.019. Epub 2018 Feb 4.
Pyrethroids, a group of insecticides with high efficiency, low toxicity and wide spectrum, are used for pest control in agriculture. Here, we administered two representative pyrethroids (cypermethrin and deltamethrin) and an equal volume of vehicle (corn oil) to the pregnant ICR mice. This study investigated the effects of cypermethrin and deltamethrin on cerebral cortex development in mice as well as possible mechanisms in proliferation and differentiation. The results showed that histopathologic change did not occurred in the cerebral cortex using Hematoxylin and Eosin staining, however, the observation of fetuses exposed to cypermethrin and deltamethrin revealed reduction of neuronal proliferation, maturation and differentiation. Moreover, cypermethrin/deltamethrin-induced apoptosis of nerve cell was significantly higher in treated groups than that in control group by using flow cytometry, Western blot and TUNEL. It was worth mentioning that the newborns exposed to cypermethrin and deltamethrin did not showed abnormal neuronal distribution. These findings suggested that prenatal cypermethrin and deltamethrin exposure impaired corticogenesis.
拟除虫菊酯是一类高效、低毒、广谱的杀虫剂，用于农业害虫防治。在此，我们给怀孕的ICR小鼠施用了两种代表性的拟除虫菊酯（氯氰菊酯和溴氰菊酯）以及等体积的赋形剂（玉米油）。本研究调查了氯氰菊酯和溴氰菊酯对小鼠大脑皮质发育的影响以及增殖和分化的可能机制。结果显示，使用苏木精和伊红染色法未在大脑皮质中观察到组织病理学变化，然而，对暴露于氯氰菊酯和溴氰菊酯的胎儿进行观察发现，神经元的增殖、成熟和分化减少。此外，通过流式细胞术、蛋白质免疫印迹法和TUNEL法检测发现，氯氰菊酯/溴氰菊酯诱导的神经细胞凋亡在处理组中显著高于对照组。值得一提的是，暴露于氯氰菊酯和溴氰菊酯的新生小鼠未表现出异常的神经元分布。这些发现表明，产前暴露于氯氰菊酯和溴氰菊酯会损害皮质发生。
