Analytical Chemistry Department, Institute of Chemistry, State University of Campinas, 13083-970 Campinas, SP, Brazil.
Analytical Chemistry Department, Institute of Chemistry, State University of Campinas, 13083-970 Campinas, SP, Brazil.
Food Chem. 2018 May 30;249:77-83. doi: 10.1016/j.foodchem.2017.12.092. Epub 2018 Jan 2.
In this work, a rapid, effective, and safe method, generating only a small amount of waste, based on the citrate version of QuEChERS was optimized and validated for multiresidue determination of pesticides of different classes in sweet green peppers, determined by liquid chromatography coupled with tandem mass spectrometry. The matrix components influenced the measurement of the pesticides by the developed analysis technique, so that, analytical curves were prepared using pesticide-free matrix extracts for quantification of the analytes. The method provides satisfactory accuracy verified by recoveries of 70-120%, and good precision (coefficients of variation ≤20%). It also showed selectivity, linearity of response, and lower limits of quantification than the maximum limit of residue for each compound, as established by ANVISA and Codex Alimentarius.
在这项工作中，优化并验证了一种基于柠檬酸版本的 QuEChERS 的快速、有效且安全的方法，该方法仅产生少量废物，可用于液相色谱-串联质谱法多残留测定甜辣椒中的不同类别农药。基质成分会影响所开发分析技术对农药的测量，因此，使用无农药基质提取物制备分析曲线来定量分析物。该方法通过回收率为 70-120%验证了令人满意的准确性，并且具有良好的精密度（变异系数≤20%）。它还表现出选择性、响应线性和低于每个化合物的最大残留限量的定量下限，这些限量是由 ANVISA 和食品法典委员会确定的。
