Gyeongnam Provincial Office, National Agricultural Products Quality Management Service, Busan 47537, Republic of Korea.
Experiment Research Institute, National Agricultural Products Quality Management Service, Gimcheon-si 39660, Republic of Korea.
Molecules. 2023 Jul 22;28(14):5589. doi: 10.3390/molecules28145589.
Pesticides effectively reduce the population of various pests that harm crops and increase productivity, but leave residues that adversely affect health and the environment. Here, a simultaneous multicomponent analysis method based on ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) pretreated by the QuEChERS method was developed to control the maximum residual levels. Among the 140 pesticides with high frequency of detection in agricultural products in Gyeongnam region in Korea for 5 years, 12 pesticides with high detection frequency in sweet pepper were selected. The analytical method is validated, linearities are r > 0.999, limit of detection (LOD) ranges from 1.4 to 3.2 µg/kg, and limit of quantification (LOQ) ranges from 4.1 to 9.7 µg/kg, and the recovery rate was 81.7-99.7%. In addition, it was confirmed that a meaningful value of these parameters can be achieved by determining the measurement uncertainty. The results proved that parameters such as recovery rate and relative standard deviation of the analysis method were within international standards. Using the developed method, better and safer sweet peppers will be provided to consumers, and effective pesticide residue management will be possible by expanding to other agricultural products.
农药能有效降低危害作物的各种害虫的种群数量，提高生产力，但会留下残留，对健康和环境造成不利影响。在这里，开发了一种基于 QuEChERS 方法预处理的超高效液相色谱四极杆飞行时间质谱（UHPLC-QTOF-MS）的同时多组分分析方法来控制最大残留水平。在韩国 5 年内对庆尚南道农产品中高频检测的 140 种农药中，选择了甜椒中高频检测的 12 种农药。对分析方法进行了验证，线性相关系数 r ＞0.999，检出限（LOD）范围为 1.4-3.2μg/kg，定量限（LOQ）范围为 4.1-9.7μg/kg，回收率为 81.7-99.7%。此外，通过测定测量不确定度，证实这些参数的有意义值可以达到。结果证明，分析方法的回收率和相对标准偏差等参数符合国际标准。通过使用所开发的方法，可以为消费者提供更好、更安全的甜椒，并通过扩展到其他农产品来实现有效的农药残留管理。
