Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana-Champaign, IL, USA.
Vet Microbiol. 2018 Feb;214:132-139. doi: 10.1016/j.vetmic.2017.12.022. Epub 2017 Dec 28.
Broad-spectrum antibiotics such as ceftiofur and ampicillin are recommended for the treatment of metritis in dairy cows. Nonetheless, little is known about the impacts of antibiotics on the uterine microbiota. Here, we evaluated the shift in uterine microbiota after treating metritic cows with ceftiofur or ampicillin, and also gained insight into the uterine microbiota associated with cure of metritis. Uterine swabs from ceftiofur-treated, ampicillin-treated, and untreated metritic Holstein cows were collected on the day of metritis diagnosis (D1) and on D6 and then used for genomic DNA extraction and sequencing of the V4 hypervariable region of the 16S rRNA gene on the Illumina MiSeq platform. The uterine microbiota consolidated over time by decreasing species richness and increasing evenness; therefore, becoming more homogeneous. The uterine microbial community showed distinct clustering patterns on D6 according to antibiotic treatment, which could be attributed to more dynamic changes in the microbial structure from D1 to D6 in ceftiofur-treated cows. Ceftiofur led to significant changes at the community level, phylum level, and genus level, whereas the changes in ampicillin and untreated cows, although following the same pattern, were mostly non-significant. Bacteroidetes was significantly increased in ceftiofur-treated cows but was not changed after ampicillin and no treatment. Different responses to antibiotics were observed in Porphyromonas, which increased in relative abundance with ceftiofur and decreased with ampicillin. Regardless of treatment group, failure to cure metritis was associated with a decrease in diversity of uterine microbiota and an increase in the relative abundance of Bacteroides, Porphyromonas, and Fusobacterium.
广谱抗生素，如头孢噻呋和氨苄西林，被推荐用于治疗奶牛子宫内膜炎。尽管如此，人们对抗生素对子宫微生物群的影响知之甚少。在这里，我们评估了头孢噻呋或氨苄西林治疗子宫内膜炎奶牛后子宫微生物群的变化，并深入了解了与子宫内膜炎治愈相关的子宫微生物群。在子宫内膜炎诊断的当天（D1）和第 6 天（D6），从头孢噻呋治疗、氨苄西林治疗和未治疗的患有子宫内膜炎的荷斯坦奶牛中采集子宫拭子，用于基因组 DNA 提取和 Illumina MiSeq 平台上 16S rRNA 基因 V4 高变区的测序。子宫微生物群随时间推移而巩固，物种丰富度降低，均匀度增加；因此，变得更加同质。根据抗生素治疗，子宫微生物群落在 D6 显示出明显的聚类模式，这可能归因于头孢噻呋治疗奶牛的微生物结构从 D1 到 D6 的更动态变化。头孢噻呋导致群落水平、门水平和属水平的显著变化，而氨苄西林和未处理牛的变化虽然遵循相同的模式，但大多不显著。厚壁菌门在头孢噻呋治疗的奶牛中显著增加，但在氨苄西林和未治疗后没有变化。在相对丰度上，头孢噻呋治疗的奶牛中卟啉单胞菌增加，而氨苄西林治疗的奶牛中减少。无论治疗组如何，未能治愈子宫内膜炎与子宫微生物群多样性的降低以及拟杆菌属、卟啉单胞菌属和梭杆菌属相对丰度的增加有关。
